This present study was an effort to explore the impact of organization culture on employee’s performance. The objectives of study were to determine the relationship between organizational culture and employee’s performance, to find out the gender based differences regarding employee’s performance and to find out the gender based differences regarding organizational culture. Role based performance and organizational culture questionnaires were used. The 20 items questionnaire relating employee’s performance and 17 items concerning organizational culture were used to collect the data for study. Sample of 60 employees were taken from different organizations. Mean, standard deviation, t-test and Pearson correlation were used for statistical analysis. The findings indicates that there is positive relationship between employees performance and organizational culture and also indicates that there is no significant difference in responses between gender of employees regarding organizational culture and employee performance. Keywords: organizational culture, employee’s performanc