Structuralism analysis be further explore the existence of characters that need to be analyzed relationships between characters in the story. Therefore, the researchers used actants theory developed by Greimas. Therefore, the researcher need to do research to discuss the topic with the following research questions: 1) How is the analysis of characterization of the main characters in Sherlock Holmes novel? 2) How is actant method and functional model applied to characters in Sherlock Holmes novel?. From the analysis of the data stated that the structuralism analysis of the main characters of The Memoirs of Sherlock Holmes novel is character of Sherlock Holmes having advantages as a smart person in revealing cases handled, but has drawbacks as a user drug. Then character of Dr. Watson had character who patient and reliable, especially in dealing with drawback Holmes. In the last, character of Professor James Moriarty is the biggest rivals for Holmes, but it also turned out to help Holmes in the use of drugs. Based on the findings of the data analysis, it can be concluded that by using actant structural analysis, it can be seen from the schema actant how the characters in the novel are interconnected with one another to create complete story