Newly synthesized cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA), p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA) and p-chloro benzyl cinnamo hydroxamic acid (p-ClBCHA), were synthesized and characterized by 1HNMR, IR spectroscopy and elemental analysis. These compounds are shows bacteristatic and bactericidal properties by the interaction of bacterial cell wall with two process bacterial cell wall synthesis inhibitors and disruption of protein synthesis. The bacterial cell growth zone of inhibition by these compounds (CHA, MCHA, N-BCHA, p-CH3BCHA, p-ClBCHA) for Lactobacillus are 13 mm, 17 mm, 14 mm, 15 mm, 22 mm and for Escherichia coli are 14 mm, 16 mm, 19 mm, 12 mm, 20 mm, at 1000 ppm respectively. Detailed analysis shows that these compounds are good cell growth of inhibition by the study of antibacterial activity against Lactobacillus and Escherichia coli bacteria. Keywords: Cinnamo hydroxamic acids, Antibacterial, Inhibition, Bacterial, Activity

Rajput, Surendra K.

English

International Institute for Science, Technology and Education (IISTE): E-Journals

Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.1, 2017  26 Antibacterial Screening of Some Newly Synthesized Cinnamo Hydroxamic Acids  Surendra K. Rajput* Principal, V. Y. T. P. G. Autonomous College, Durg (C.G.), 492010, INDIA  Anita Patel Department of chemistry,Govt.  N. P.G. College of Science, Raipur, (C.G.) 492010, INDIA.  Kishor N. Bapat Additional Director, Directorate of Higher Education Raipur (C.G), INDIA.  Abstract Newly synthesized cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA), p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA) and p-chloro benzyl cinnamo hydroxamic acid (p-ClBCHA), were synthesized and characterized by 1HNMR, IR spectroscopy and elemental analysis. These compounds are shows bacteristatic and bactericidal properties by the interaction of bacterial cell wall with two process bacterial cell wall synthesis inhibitors and disruption of protein synthesis. The bacterial cell growth zone of inhibition by these compounds (CHA, MCHA, N-BCHA, p-CH3BCHA, p-ClBCHA) for Lactobacillus  are 13 mm, 17 mm, 14 mm, 15 mm, 22 mm and for Escherichia coli are 14 mm, 16 mm, 19 mm, 12 mm, 20 mm, at 1000 ppm respectively. Detailed analysis shows that these compounds are good cell growth of inhibition by the study of antibacterial activity against Lactobacillus and Escherichia coli bacteria. Keywords: Cinnamo hydroxamic acids, Antibacterial, Inhibition, Bacterial, Activity.                                      1. Introduction Hydroxamic acids refer to a class of chemical organic compounds having formula RC (=O) NR’OH in which hydroxylamine is inserted in to a carboxylic acids with ‘R’ as an organic residue (alkyl or aryl group), a CO as a carbonyl group, and a hydroxylamine as NH2-OH [1][Figure 1].    Figure1. General structure of hydroxamic acid, where R as an alkyl and aryl group. The subject of this research is to study the antibacterial activity of synthesized cinnamo hydroxamic acids against Lactobacillus and Escherichia coli.  Lactobacillus may also cause arthritis, bloating, blockage of a lung artery, diarrhea, disease of the esophagus, heart inflammation, liver infection, skin reactions, stomach cramping, stomach lining inflammation, stomach rumbling, and vaginal burning and discomfort[2]. Escherichia coli, usually called E. Coli, refers to a large group of bacteria that is commonly found in the intestines of humans and animals, Escherichia coli may also causing severe stomach cramps, diarrhea and vomiting [3]. Cinnamo hydroxamic acids are commonly synthesized by acylation of hydroxylamine and cinnamoyl chloride [4] .When a hydroxamic acid has got achieved then it got gift to many fields like –pharmaceutical[5], medicinal biological[6], medical molecular modeling[7], analytical[8] and nuclear chemistry[9], as well as their role as antibacterial [10], antifungal[11], antitumor[12] and  anti-inflammatory activities[13]. One of the most important activity of cinnamo hydroxamic acids are ability to inhibit various enzyme viz- peroxidase[14],  urease[15]  or  ribonucleotide reductase[16], metalloproteinase[17] and hydrolases[18]. Mechanism of enzyme inhibition for cinnamo hydroxamic acids by electophilic character, the electrophilicity allows the molecule to react with nucleophilic center present in enzyme involved in bacterial cell wall [19]. In view of the above application, the present work relates to the synthesis and characterization of cinnamo hydroxamic acids such as cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA), p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA), p-chloro benzyl cinnamo hydroxamic acid (p-ClBCHA), and reports the results of the undertaken antibacterial evaluation against Lactobacillus and Escherichia coli.   2. Materials and instrumentation All reagents and chemicals were purchased from Sigma Aldrich and Merck products used. Cinnamo hydroxamic acids were synthesized and purified according to the literature procedure. All the glass wares were cleaned with chromic acids cleaning solution followed by distilled water. These were then sterilized and stored in dust proof Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.1, 2017  27 cabinets. The bacterial cultures Lactobacillus and Escherichia coli used were 5-7 days olds. For the preparation of standard nutrient agar medium beef extract 3 gm, peptone 5 gm, agar-agar  15 gm, NaCl 5gm, congored 2.5ml and distilled water 1000ml was used for determination of antibacterial activity.   2.1 Preparation of media Beef extract 3 gm, peptone 5 gm, agar-agar  15 gm, NaCl 5gm, congored 2.5ml  are mixed and boiled in 500 ml distilled water for one hour filtered and volume made to 1000 ml by distilled water. The medium was auto claved at prior half an hour [20].  2.2 Preparation of  bio disc  Preparation of biodisc, multiple layers of whatman filter paper (no.42) carefully cut in 1cm diameter are used and prepared of the above five synthesized cinnamo hydroxamic acid derivatives in the concentration level of 1000 µg/ml using DMSO (di methyl sulphoxide) [21].  2.3 Preparation of standard solution The antibacterial activity of each compound was evaluated at 1000ppm, 100ppm, 10ppm, concentrations.                                                                                                              Three solution of different concentration with media (1000ppm, 100ppm, 10ppm,) were prepared with the help of the following equation making use of stock solution [22].     2.4 Procedure for Antibacterial activity Newly prepared compounds were screened for their activity against  Lactobacillus and Escherichia coli  in DMSO by paper disk diffusion method the prepared 50 ml nutrient ager medium (NAM) taken in a number of 100 ml conical flask were plugged with cotton autoclaved for half an hour at 20 psi pressure. Normal saline was used to make a suspension of spore of fungal strain was transferred to 30 ml saline to get a suspension of corresponding species. Agar media (20 ml) were powered in to each species. Excess of suspension was decanted and plates were dried by placing in incubation at 72 hrs at 28± oC in culture room. The testing was repeated three times for each concentration of the compound under investigations, along with affair number of replicates of the control plates [23].   3. Result and discussion  Activities of each compound were observed and compared with tetracycline used as standard drugs. The bacterial colony diameter was measured at 24, 48 and 72 hrs in three diameters by millimeter scale. The diameters were marked by pencil for subsequent identification. The inhibition of the bacterial growth was determined as the difference in growth between test and control plates (Table. 1). The percentage inhibition in colony of the test bacteria was expressed as:-       3.1 Zone of inhibition of Lactobacillus and  The synthesized compounds and the reference drugs were screened under identical conditions and the zone of inhibition was measured in mm. The study of antibacterial activity showed that p-ClBCHA was highly sensitive for Lactobacillus against cinnano hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA) and p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA), derivatives of hydroxamic acids (Table 1).   3.2 Zone of inhibition of E. coli  The synthesized compounds and the reference drugs were screened under identical conditions and the zone of inhibition was measured in mm. The study of antibacterial activity showed that p-ClBCHA was also highly sensitive for Escherichia coli against cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA) and p-methyl benzyl cinnamo hydroxamic acid (p-Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.1, 2017  28 CH3BCHA), derivatives of hydroxamic acids (Table 1).  4. Conclusion  In this work, the method used for the synthesis of final compounds in the manuscript is very useful in various field of analytical, pharmaceutical, medical molecular modeling, docking and nuclear chemistry. The compounds are very big antibacterial activity and are selective inhibitors of various enzymes. The antibacterial screening data showed a moderate to good activity at higher concentration but bacteriocity decreases considerably upon dilution p-ClBCHA are more toxic to Lactobacillus and Escherichia coli (Table-2), The sequence of antibacterial activity of these compounds for Lactobacillus are p-ClBCHA>MCHA>p-CH3BCHA>N-BCHA>CHA and for Escherichia coli p-ClBCHA>N-BCHA> MCHA>CHA>p-CH3BCHA at higher concentration 1000pm respectively (Figure 2).  5. Acknowledgements Thanks to my supervisor Dr. S. K. Rajput, co-supervisor Dr. K. N. Bapat, thanks to department of chemistry, botany, Govt.  N.  P. G. College of Science, Raipur, and thanks to department of chemistry, Gujarat University Ahmedabad  Gujarat for IR analysis and SAIF Punjab University Chandigarh for 1HNMR analysis Thanks to CIL Punjab University Chandigarh for Elemental analysis .  References 1. Kakkar R., Gupta S. P.( 2013). Theoretical studies on hydroxamic acids. Springer-Verlage Berlin Heidelberg, 19. 2. Bahri F., Lejeune A., Dubois-Dauphin R., Elmejdoub T., Boulahrouf A., Thonart P. (2014). Characterization of Lactobacillus strains isolated from Algerian children faeces for their probiotic properties. African J. Micro. Res., 8, 297-303. 3. Bahiru A.A., Emire S. A., Ayele A. K. (2013).The prevalence of antibiotic resistant Escherichia coli isolates from fecal and water sources. Academia J. Micro. Res., 1, 1-10. 4. Rajput S. K., Patel A., Bapat K. N. (2016). Synthesis, characterization and spectrophotometric determination of vanadium (V) with unsubstituted cinnamo hydroxamic acid. Asian J. Chem., 29, 465-468. 5. Pravin N., Raman N. (2013). DNA interaction and antimicrobial activity of novel tetradentateiminooxalato mixed ligand metal complexes. Inorg. Chem. Commun., 36, 45-50. 6. Abualreish M. J. A., Abdein M. A. (2014). The analytical applications and biological activity of hydroxamic acids. J. Adv. Chem., 10, 2118-2125. 7. Saha S., Banerjee S., Ganguly S. (2010). Molecular docking studies of some novel hydroxamic acid derivatives. Inter. J. chem. Tech. Res., 2, 932-936. 8. Rajput S. K., Patel A., Bapat K. N. (2016). Spectrophotometric determination of vanadium (V) using N–methyl cinnamo hydroxamic acid as reagent. Chem. Mat. Res., 8, 8-16.  9.  Agrawal S., Khan F., Ganesh S. (2012). Spectrophotometric studies on chelating behavior of oxalohydroxamic acid with uranium in aqueous streams. Chem. Mat. Res., 2, 58-63, 10. Shankar B., Tomar R., Kumar R., Godhara M., Sharma V. K. (2014). Antimicrobial activity of newly synthesized hydroxamic acid of pyrimidine-5-carboxylic acid and its complexes with Cu(II), Ni(II), Co(II) and Zn(II) metal ions. J. Chem. Pharma. Res., 6,925-930 11. Kabeer A. S., Baseer M. A., Mote N. A. (2001). Synthesis and antimicrobial activity of some schiff bases from benzothiazoles. Asian J. Chem., 13, 496-500. 12. Pontiki E., Hadjipavlou-Litina D., Litinas K. Geromichalos G. (2014). Novel cinnamic acid derivatives as   antioxidant and anticancer agents: design, synthesis and modeling studies. Mole., 19, 9655-9674. 13. Raji Z., Butula I.,  Zorc B., Kraljevi S., Hock K., Paveli K., Naesens L., De C. E., Balzarini J., Przyborowska M., Ossowski T., Mintas M. (2009). Cytostatic and antiviral evaluations of hydroxamic derivatives of some nonsteroidal anti-inflammatorydrugs. Chem. Biol. Drug. Des., 73, 328-338. 14. Indiani C., Santoni E., Becucci M., Boffi A., Fukuyama K., Smulevich G. (2003). New insight into the peroxidasehydroxamic acid interaction  revealed by the combination of spectroscopic and crystallographic studies. Biochem., 47,14066-14074. 15.  Amtul Z., Ur-Rahman A., Siddiqui R. A., Choudhary M. I. (2002). Chemistry and mechanism of urease inhibition.  Curr. Med. Chem., 26, 1323- 1248. 16.  Shao J., Zhou B., Chu B.,Yen Y. Ribonucleotide Reductase Inhibitors and Future Drug Design.(2006). Current Cancer Drug Targets, 6, 409-431.  17. Sani M., Belotti D., Giavazzi R., Panzeri W., Volonterio A., Zanda M. (2004). Synthesis and evaluation of stereopure α-trifluoromethyl-malic hydroxamates as inhibitors of matrix metalloproteinases.Tetrahedron , 45, 1611-1615.  Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.1, 2017  29 18. Brown D. A., Cuffe L. P., Fitzpatrick N. J., Ryan Á. T. (2003). A DFT study of model complexes of zinc hydrolases and their inhibition by hydroxamic acids. Inorg. Chem., 43, 297-302,  19. Michael A., Kohansk, Daniel J., Dwyer, Collins J. J.(2010). How antibiotics kill bacteria: from targets to networks.  Nat. Rev., 8, 423-435. 20. Krishnan R. (2012). Synthesis, kinetic study and biological activity of some heterocyclic hydroxamic acids. Thesis pt. R. S. U. Raipur (C.G.). 21. Shekhawat S. S. (2012). Synthesis, kinetic study and antimicrobial activities of some thiophene hydroxamic acids. Thesis pt. R. S. U. Raipur (C.G.). 22. Raghavan V. (2006). Synthesis, kinetic study and antimicrobial activity of some heterocyclic hydroxamic acids. Thesis pt. R. S. U. Raipur (C.G.). 23. Rangel P., Marin J. M. (2009). Analysis of Escherichia coli isolated from fecal samples. Pesquisa veterinária brasileiral, 29, 363-368.  Table-1. Following compounds have been evaluated for their antibacterial activity .  Figure 2. Cinnamo hydroxamic acid derivatives compound have been evaluated for their antibacterial activity against Lactobacillus and Escherichia coli.    S. No.   Name of   compound Average percentage inhibition after organism  Lactobacillus concentration used Average percentage inhibition after organism  Escherichia coli  Concentration used 1000ppm 100ppm 10ppm 1000ppm 100ppm 10ppm 1. CHA 13 11 9 14 11 8 2. MCHA 17 15 12 16 15 13 3. N-BCHA 14 12 11 19 16 14 4. p-CH3BCHA 15 13 10 12 10 9 5. p-ClBCHA 22 18 16 20 17 15 6. Tetracycline(Standard) 55 50 32 44 39 31 

Antibacterial Screening of Some Newly Synthesized Cinnamo Hydroxamic Acids

Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.2, 2017  14 Antibacterial Screening of Some Newly Synthesized Cinnamo Hydroxamic Acids  Surendra K. Rajput* Principal, V. Y. T. P. G. Autonomous College, Durg (C.G.), 492010, INDIA  Anita Patel Department of chemistry,Govt.  N. P.G. College of Science, Raipur, (C.G.) 492010, INDIA  Kishor N. Bapat Additional Director, Directorate of Higher Education Raipur (C.G), INDIA  Abstract Newly synthesized cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA), p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA) and p-chloro benzyl cinnamo hydroxamic acid (p-ClBCHA), were synthesized and characterized by 1HNMR, IR spectroscopy and elemental analysis. These compounds are shows bacteristatic and bactericidal properties by the interaction of bacterial cell wall with two process bacterial cell wall synthesis inhibitors and disruption of protein synthesis. The bacterial cell growth zone of inhibition by these compounds (CHA, MCHA, N-BCHA, p-CH3BCHA, p-ClBCHA) for Lactobacillus  are 13 mm, 17 mm, 14 mm, 15 mm, 22 mm and for Escherichia coli are 14 mm, 16 mm, 19 mm, 12 mm, 20 mm, at 1000 ppm respectively. Detailed analysis shows that these compounds are good cell growth of inhibition by the study of antibacterial activity against Lactobacillus and Escherichia coli bacteria. Keywords: Cinnamo hydroxamic acids, Antibacterial, Inhibition, Bacterial, Activity.                                                                         1. Introduction Hydroxamic acids refer to a class of chemical organic compounds having formula RC (=O) NR’OH in which hydroxylamine is inserted in to a carboxylic acids with ‘R’ as an organic residue (alkyl or aryl group), a CO as a carbonyl group, and a hydroxylamine as NH2-OH [1][Figure 1].    Figure1. General structure of hydroxamic acid, where R as an alkyl and aryl group. The subject of this research is to study the antibacterial activity of synthesized cinnamo hydroxamic acids against Lactobacillus and Escherichia coli.  Lactobacillus may also cause arthritis, bloating, blockage of a lung artery, diarrhea, disease of the esophagus, heart inflammation, liver infection, skin reactions, stomach cramping, stomach lining inflammation, stomach rumbling, and vaginal burning and discomfort[2]. Escherichia coli, usually called E. Coli, refers to a large group of bacteria that is commonly found in the intestines of humans and animals, Escherichia coli may also causing severe stomach cramps, diarrhea and vomiting [3]. Cinnamo hydroxamic acids are commonly synthesized by acylation of hydroxylamine and cinnamoyl chloride [4] .When a hydroxamic acid has got achieved then it got gift to many fields like –pharmaceutical[5], medicinal biological[6], medical molecular modeling[7], analytical[8] and nuclear chemistry[9], as well as their role as antibacterial [10], antifungal[11], antitumor[12] and  anti-inflammatory activities[13]. One of the most important activity of cinnamo hydroxamic acids are ability to inhibit various enzyme viz- peroxidase[14],  urease[15]  or  ribonucleotide reductase[16], metalloproteinase[17] and hydrolases[18]. Mechanism of enzyme inhibition for cinnamo hydroxamic acids by electophilic character, the electrophilicity allows the molecule to react with nucleophilic center present in enzyme involved in bacterial cell wall [19]. In view of the above application, the present work relates to the synthesis and characterization of cinnamo hydroxamic acids such as cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA), p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA), p-chloro benzyl cinnamo hydroxamic acid (p-ClBCHA), and reports the results of the undertaken antibacterial evaluation against Lactobacillus and Escherichia coli.   2. Materials and instrumentation All reagents and chemicals were purchased from Sigma Aldrich and Merck products used. Cinnamo hydroxamic acids were synthesized and purified according to the literature procedure. All the glass wares were cleaned with chromic acids cleaning solution followed by distilled water. These were then sterilized and stored in dust proof cabinets. The bacterial cultures Lactobacillus and Escherichia coli used were 5-7 days olds. For the preparation Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.2, 2017  15 of standard nutrient agar medium beef extract 3 gm, peptone 5 gm, agar-agar  15 gm, NaCl 5gm, congored 2.5ml and distilled water 1000ml was used for determination of antibacterial activity.   2.1 Preparation of media Beef extract 3 gm, peptone 5 gm, agar-agar  15 gm, NaCl 5gm, congored 2.5ml  are mixed and boiled in 500 ml distilled water for one hour filtered and volume made to 1000 ml by distilled water. The medium was auto claved at prior half an hour [20].  2.2 Preparation of  bio disc  Preparation of biodisc, multiple layers of whatman filter paper (no.42) carefully cut in 1cm diameter are used and prepared of the above five synthesized cinnamo hydroxamic acid derivatives in the concentration level of 1000 µg/ml using DMSO (di methyl sulphoxide) [21].  2.3 Preparation of standard solution The antibacterial activity of each compound was evaluated at 1000ppm, 100ppm, 10ppm, concentrations.                                                                                                              Three solution of different concentration with media (1000ppm, 100ppm, 10ppm,) were prepared with the help of the following equation making use of stock solution [22].     2.4 Procedure for Antibacterial activity Newly prepared compounds were screened for their activity against  Lactobacillus and Escherichia coli  in DMSO by paper disk diffusion method the prepared 50 ml nutrient ager medium (NAM) taken in a number of 100 ml conical flask were plugged with cotton autoclaved for half an hour at 20 psi pressure. Normal saline was used to make a suspension of spore of fungal strain was transferred to 30 ml saline to get a suspension of corresponding species. Agar media (20 ml) were powered in to each species. Excess of suspension was decanted and plates were dried by placing in incubation at 72 hrs at 28± oC in culture room. The testing was repeated three times for each concentration of the compound under investigations, along with affair number of replicates of the control plates [23].   3. Result and discussion  Activities of each compound were observed and compared with tetracycline used as standard drugs. The bacterial colony diameter was measured at 24, 48 and 72 hrs in three diameters by millimeter scale. The diameters were marked by pencil for subsequent identification. The inhibition of the bacterial growth was determined as the difference in growth between test and control plates (Table. 1). The percentage inhibition in colony of the test bacteria was expressed as:-        3.1 Zone of inhibition of Lactobacillus and  The synthesized compounds and the reference drugs were screened under identical conditions and the zone of inhibition was measured in mm. The study of antibacterial activity showed that p-ClBCHA was highly sensitive for Lactobacillus against cinnano hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA) and p-methyl benzyl cinnamo hydroxamic acid (p-CH3BCHA), derivatives of hydroxamic acids (Table 1).   3.2 Zone of inhibition of E. coli  The synthesized compounds and the reference drugs were screened under identical conditions and the zone of inhibition was measured in mm. The study of antibacterial activity showed that p-ClBCHA was also highly sensitive for Escherichia coli against cinnamo hydroxamic acids (CHA), N-methyl cinnamo hydroxamic acid (MCHA), N-benzyl cinnamo hydroxamic acid (N-BCHA) and p-methyl benzyl cinnamo hydroxamic acid (p-Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.2, 2017  16 CH3BCHA), derivatives of hydroxamic acids (Table 1).  4. Conclusion  In this work, the method used for the synthesis of final compounds in the manuscript is very useful in various field of analytical, pharmaceutical, medical molecular modeling, docking and nuclear chemistry. The compounds are very big antibacterial activity and are selective inhibitors of various enzymes. The antibacterial screening data showed a moderate to good activity at higher concentration but bacteriocity decreases considerably upon dilution p-ClBCHA are more toxic to Lactobacillus and Escherichia coli (Table-2), The sequence of antibacterial activity of these compounds for Lactobacillus are p-ClBCHA>MCHA>p-CH3BCHA>N-BCHA>CHA and for Escherichia coli p-ClBCHA>N-BCHA> MCHA>CHA>p-CH3BCHA at higher concentration 1000pm respectively (Figure 2).  5. Acknowledgements Thanks to my supervisor Dr. S. K. Rajput, co-supervisor Dr. K. N. Bapat, thanks to department of chemistry, botany, Govt.  N.  P. G. College of Science, Raipur, and thanks to department of chemistry, Gujarat University Ahmedabad  Gujarat for IR analysis and SAIF Punjab University Chandigarh for 1HNMR analysis Thanks to CIL Punjab University Chandigarh for Elemental analysis .  References 1. Kakkar R., Gupta S. P.( 2013). Theoretical studies on hydroxamic acids. Springer-Verlage Berlin Heidelberg, 19. 2. Bahri F., Lejeune A., Dubois-Dauphin R., Elmejdoub T., Boulahrouf A., Thonart P. (2014). Characterization of Lactobacillus strains isolated from Algerian children faeces for their probiotic properties. African J. Micro. Res., 8, 297-303. 3. Bahiru A.A., Emire S. A., Ayele A. K. (2013).The prevalence of antibiotic resistant Escherichia coli isolates from fecal and water sources. Academia J. Micro. Res., 1, 1-10. 4. Rajput S. K., Patel A., Bapat K. N. (2016). Synthesis, characterization and spectrophotometric determination of vanadium (V) with unsubstituted cinnamo hydroxamic acid. Asian J. Chem., 29, 465-468. 5. Pravin N., Raman N. (2013). DNA interaction and antimicrobial activity of novel tetradentateiminooxalato mixed ligand metal complexes. Inorg. Chem. Commun., 36, 45-50. 6. Abualreish M. J. A., Abdein M. A. (2014). The analytical applications and biological activity of hydroxamic acids. J. Adv. Chem., 10, 2118-2125. 7. Saha S., Banerjee S., Ganguly S. (2010). Molecular docking studies of some novel hydroxamic acid derivatives. Inter. J. chem. Tech. Res., 2, 932-936. 8. Rajput S. K., Patel A., Bapat K. N. (2016). Spectrophotometric determination of vanadium (V) using               N–methyl cinnamo hydroxamic acid as reagent. Chem. Mat. Res., 8, 8-16.  9.  Agrawal S., Khan F., Ganesh S. (2012). Spectrophotometric studies on chelating behavior of oxalohydroxamic acid with uranium in aqueous streams. Chem. Mat. Res., 2, 58-63, 10. Shankar B., Tomar R., Kumar R., Godhara M., Sharma V. K. (2014). Antimicrobial activity of newly synthesized hydroxamic acid of pyrimidine-5-carboxylic acid and its complexes with Cu(II), Ni(II), Co(II) and Zn(II) metal ions. J. Chem. Pharma. Res., 6,925-930 11. Kabeer A. S., Baseer M. A., Mote N. A. (2001). Synthesis and antimicrobial activity of some schiff bases from benzothiazoles. Asian J. Chem., 13, 496-500. 12. Pontiki E., Hadjipavlou-Litina D., Litinas K. Geromichalos G. (2014). Novel cinnamic acid derivatives as   antioxidant and anticancer agents: design, synthesis and modeling studies. Mole., 19, 9655-9674. 13. Raji Z., Butula I.,  Zorc B., Kraljevi S., Hock K., Paveli K., Naesens L., De C. E., Balzarini J., Przyborowska M., Ossowski T., Mintas M. (2009). Cytostatic and antiviral evaluations of hydroxamic derivatives of some nonsteroidal anti-inflammatorydrugs. Chem. Biol. Drug. Des., 73, 328-338. 14. Indiani C., Santoni E., Becucci M., Boffi A., Fukuyama K., Smulevich G. (2003). New insight into the peroxidasehydroxamic acid interaction  revealed by the combination of spectroscopic and crystallographic studies. Biochem., 47,14066-14074. 15.  Amtul Z., Ur-Rahman A., Siddiqui R. A., Choudhary M. I. (2002). Chemistry and mechanism of urease inhibition.  Curr. Med. Chem., 26, 1323- 1248. 16.  Shao J., Zhou B., Chu B.,Yen Y. Ribonucleotide Reductase Inhibitors and Future Drug Design.(2006). Current Cancer Drug Targets, 6, 409-431.  17. Sani M., Belotti D., Giavazzi R., Panzeri W., Volonterio A., Zanda M. (2004). Synthesis and evaluation of stereopure α-trifluoromethyl-malic hydroxamates as inhibitors of matrix metalloproteinases.Tetrahedron , 45, 1611-1615.  18. Brown D. A., Cuffe L. P., Fitzpatrick N. J., Ryan Á. T. (2003). A DFT study of model complexes of zinc Chemistry and Materials Research                                                                                                                                                    www.iiste.org ISSN 2224- 3224 (Print) ISSN 2225- 0956 (Online) Vol.9 No.2, 2017  17 hydrolases and their inhibition by hydroxamic acids. Inorg. Chem., 43, 297-302,  19.  Michael A., Kohansk, Daniel J., Dwyer, Collins J. J.(2010). How antibiotics kill bacteria: from targets to networks.  Nat. Rev., 8, 423-435. 20. Krishnan R. (2012). Synthesis, kinetic study and biological activity of some heterocyclic hydroxamic acids. Thesis pt. R. S. U. Raipur (C.G.). 21. Shekhawat S. S. (2012). Synthesis, kinetic study and antimicrobial activities of some thiophene hydroxamic acids. Thesis pt. R. S. U. Raipur (C.G.). 22. Raghavan V. (2006). Synthesis, kinetic study and antimicrobial activity of some heterocyclic hydroxamic acids. Thesis pt. R. S. U. Raipur (C.G.). 23. Rangel P., Marin J. M. (2009). Analysis of Escherichia coli isolated from fecal samples. Pesquisa veterinária brasileiral, 29, 363-368.  Table-1. Following compounds have been evaluated for their antibacterial activity .   S. No.   Name of   compound Average percentage inhibition after organism  Lactobacillus concentration used Average percentage inhibition after organism  Escherichia coli  Concentration used 1000ppm 100ppm 10ppm 1000ppm 100ppm 10ppm 1. CHA 13 11 9 14 11 8 2. MCHA 17 15 12 16 15 13 3. N-BCHA 14 12 11 19 16 14 4. p-CH3BCHA 15 13 10 12 10 9 5. p-ClBCHA 22 18 16 20 17 15 6. Tetracycline(Standard) 55 50 32 44 39 31  Figure 2. Cinnamo hydroxamic acid derivatives compound have been evaluated for their antibacterial activity against Lactobacillus and Escherichia coli.    

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Antibacterial Screening of Some Newly Synthesized Cinnamo Hydroxamic Acids

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