The Pepper extract acts as a reducing and capping agent in the formation of silver nanoparticles. A UV–Vis spectrum of the aqueous medium containing silver nanoparticles demonstrated a peak at 480 nm corresponding to the Plasmon absorbance of rapidly synthesized silver nanoparticles that was characterize by UV–Visible spectrophotometer.The Antibacterial testing of Pepper extract was evaluate by disk diffusion method using bacteria like Staphylococcus aureus, Bacillus sp., Escherichia coli, and Pseudomonas sp. Study revealed the antibacterial activity of solvent extracts by using pepper; we prepared the silver nanoparticles to enhance its antibacterial property.Antibacterial disc were prepared in five different concentrations such as 100?l, 200?l, 300?l, 400?l and 500?l respectively. The antimicrobial activity of particles showed higher activity in concentration 500?l against the pathogenic Pseudomonas sp. (15) mm of inhibition zone. The higher activity was followed by Bacillus sp. (13) mm of inhibition zone, Staphylococcus sp. (11) mm of inhibition zone and E. coli (10) mm of inhibition zone. This study showed that the extract reveals the concentration dependent activity. The study revealed that the silver nanoparticles from plant extracts could be as a therapeutic agent for human microbial infections. The zone of inhibition seems in both gram positive and gram negative bacterial strains. This study reports that synthesis is useful to avoid toxic chemicals with adverse effects in medical applications rather than physical and chemical methods.Thus, the aim of the study to synthesize silver based bionanoparticles. The present investigations revealed with the isolation and monitoring of silver nanoparticles to produce novel drugs to overcome drug resistance and advance reaction. Keywords: Nanoparticles, Silver, Antimicrobial activity, Nano biosynthesis, standard disc diffusion method
