16S-raw-.001-abundance.representative.sequences

Abstract

This fasta file includes 16S sequence data from different environmental samples. Within QIIME2, DADA2 was used to remove primer sequences from the reads, perform error correction, identify sequence variants, and remove chimeric sequences. Sequence variants were then classified by a naive Bayes classifier within QIIME2 using release of 128 of the Silva SSU database. An abundance filter was applied, and sequence variants that did not compose at least 0.1% of at least one library were removed from all libraries. The fasta file includes all variants that passed the abundance filter

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