Metal content in protoplasts data

Abstract

Mitochondrial metals (Fe, Mn) content in protoplasts prepared from Arabidopsis thaliana suspension cells and transformed with empty vector pA7 or with the vector carrying cucumber CsMTP6 gene. Following transformation, protoplasts were incubated with 100 mcroM FeSO4-EDTA or 100 microM MnSO4 with slow agitation. Metal content was determined by AAS.The data are expressed in nmol of metal x mg-1 protein and were obtained in three separate experiment

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