The fact that α-biotin serves as a covalently bound "C0_2 carrier" is now unequivocally established. Though model studies such as carboxylation on ureid l\u27-N-of biotin has been reported, no appropriate biomimetic model study for a "C0_2 carrier" has been carried out. The titled compound(I)in which the biotinyl group is bound to an amide linkage withβ-cyclodextrin, was prepared with active-ester method in good yield. Compound I was purified by taking with preparative HPLC. The structure of (I) was providedby ^1H-nmr, ^C-nmr, elemental analysis, HPLC and the paper choromatography of hydrolyzates, and decided to be 6-(mono-N-biotinyl)-amino-β-cyclodextrin
