The poultry industry is challenged by microbial infections, but the use of antibiotic growth promoters is restricted in many countries. This study is designed to assess the use of Ocimum gratissimum for the control of pathogenic gut microflora (E. coli and Salmonella sp). One hundred day old (ANAK 2000) commercial broilers were purchased and used for the experiment. The birds were brooded for seven days before random distribution to their respective treatments and replicates. Aqueous Ocimum gratissimum (0.11g/l) was administered to one set of 50 birds for 7 days (ocimum treatment), while the second sets of 50 birds which were not given the Ocimum extracts served as the control. Result show that the herb had a crude protein content of 4.56%, carbohydrate 13.40% and dry matter of 78.63%, but low in crude fibre (1.06%) and ash (1.21%). Prior to the administration of Ocimum extract the population of Salmonella was highest at the ileum (2.05 log cfu/g) followed by the crop (1.79 log cfu/g) and least at the caecum (1.77 log cfu/g). E. coli was highest at the ileum (2.05 log cfu/g), followed by the caecum (1.93 log cfu/g) and least at the crop (1.83 log cfu/g). Lactobacillus followed the pattern of E. coli but with different population, being highest at the ileum (1.95 log cfu/g) flowed by the caecum (1.90 log cfu/g) and least at the crop (1.79 log cfu/g). One week after the administration of Ocimum extracts to the chickens, the population of microbes in the gut decreased significantly (P<0.05) in relation to the control, suggesting the efficacy of Ocimum at reducing the population of enteric bacteria
