Livestock buildings are identified to be a major source of ammonia emissions. About 30% of the total ammonia emission within livestock sectors is from naturally ventilated dairy cattle buildings. The main objectives of this study are to predict emissions from naturally ventilated dairy cattle buildings and to establish a systematic approach to curtail the emissions.Gas concentrations were measured inside two dairy cattle buildings in mid-Jutland, Denmark. CO2 balance method was thus applied to estimate ventilation and emission rates. Computational fluid dynamics (CFD) was used to find the optimum gas sampling positions for outlet CO2 concentration. The gas sampling positions should be located adjacent to the openings or even in the openings. The NH3 emission rates varied from 32 to77 g HPU-1 d-1 in building 1 and varied from 18 to30 g HPU-1 d-1 in building 2.Scale model experiment showed that partial pit ventilation was able to remove a large portion of polluted gases under the slatted floor. In the full scale simulations, a pit exhaust with a capacity of 37.3 m3 h-1 HPU-1 may reduce ammonia emission only by 3.16% compared with the case without pit ventilation. When the external wind was decreased to 1.4 m s-1 and the sidewall opening area were reduced to half, such a pit ventilation capacity can reduce ammonia emission by 85.2%. The utilization of pit ventilation system must be integrated with the control of the natural ventilation rates of the building