The purpose of this study was to investigate the characteristic of the physical fitness of female students who specialize in health and sports by the new physical fitness test which the Ministry of Education, Science, Sports and Culture made. The subjects were 96 university female students who belong to the physical education major of this university. The results were summarized as follows: 1) The average value with total point of the new physical fitness test was 51.7 in 60 perfect scores and the physical fitness of 85.9% of students was evaluated by the A step. 2) The point with the items, which measure endurance capacity, was significantly higher than that with the items, which measure muscular strength and power. 3) Significant correlation coefficient was observed between the present sport activity, the self evaluation to the physical fitness and the endurance capacity. These results suggested that at the female students, who specialize in health and sports, the level of the total physical fitness was high, especially, the endurance capacity is excellent and the cardiovascularendurance was influenced by the present sport activity and the self evaluation to the physical fitness