Alessandra Bendini, Rosa Palagano, Enrico Valli, Maria del Carmen Perez-Camino, Wenceslao Moreda, Tullia Gallina Toschi

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Alessandra Bendini

Rosa Palagano

Enrico Valli

Maria del Carmen Perez-Camino

Wenceslao Moreda

Tullia Gallina Toschi

Archivio istituzionale della ricerca - Alma Mater Studiorum Università di Bologna

  148 Olive Oil, Tuesday 22 October 2019, 16:40-17:00, España 1 OLEUM: A European Funded Project In-house Validation of Two Analytical Protocols for Time-saving Alternative Determination of Fatty Acid Ethyl Esters in Virgin Olive Oils Alessandra Bendini1, Rosa Palagano1, Enrico Valli1, Maria del Carmen Perez-Camino2, Wenceslao Moreda2, Tullia Gallina Toschi1 1Alma Mater Studiorum - University of Bologna, Cesena, Italy 2Instituto de la Grasa (CSIC), Sevilla, Spain  The analytical evaluation of the content of fatty acids ethyl esters (FAEEs) is one of the quality parameters that permits to define the quality grade of a virgin olive oil and to detect possible fraudulent mixtures of extra virgin olive oils with lower quality oils (e.g. some kinds of soft-deodorized oils). The official method (reported in the EU Reg. 61/2011) is based on the addition of a suitable internal standard to the oil and fractionation by chromatography on a hydrated silica gel column. After the recovery of the eluted fraction, this is analyzed by capillary gas chromatography. However, it should be pointed out that the official method requires high volume of solvents and a very long preparative procedure. For this reason, one of the objectives of the OLEUM project is to propose, at least, one revised quicker and more sustainable in-house validated method for the FAEEs determination. In this work, two different approaches, by HPLC-UV-Vis or SPE, as rapid alternative to the traditional liquid chromatography applied in the preparative phase, are presented. The collected fraction containing the FAEEs are successively analyzed by capillary GC-FID with PTV or split injector, as possible options to the less widespread on-column system. Performance (e.g. intra-day precision, accuracy, linearity, LOD and LOQ, recovery and robustness) related to the in-house validation for both the revised methods is shown.  This work is developed in the context of the project OLEUM 'Advanced solutions for assuring authenticity and quality of olive oil at global scale', funded by the European Commission within the Horizon 2020 Programme (2014-2020, GA no. 635690). The information expressed in this abstract reflects the authors' views; the EC is not liable for the information contained therein. 148 OILS, FATS AND LIPIDS: DRIVING SCIENCE AND TECHNOLOGY TO NEW HORIZONS

In-house Validation of Two Analytical Protocols for Time-saving Alternative Determination of Fatty Acid Ethyl Esters in Virgin Olive Oils

https://cris.unibo.it/retrieve/handle/11585/702571/498945/Bendini%20et%20al..pdf

In-house Validation of Two Analytical Protocols for Time-saving Alternative Determination of Fatty Acid Ethyl Esters in Virgin Olive Oils

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Archivio istituzionale della ricerca - Alma Mater Studiorum Università di Bologna