Objective: To evaluate resistant trend of multiple antimicrobial agents against Staphylococcus aureus isolates along with its prevalence. Material and Methods: This prospective descriptive study was carried out in the department of microbiology Allama Iqbal Medical College Lahore from 1st January 2015 to 25 may 2016. Simple random technique was used, and 4570 clinical samples (Pus, blood, pleural fluid, tracheal aspirate, urine, sputum, HVS) were received from ICU, Surgical Unit, Medical Unit and OPD for culture sensitivity testing, antimicrobial resistant trend was tested according to CLSI guidelines Results: Maximum isolates were recovered from surgical unit 31.9%, sample-wise maximum isolates were recovered from pus samples 26.8%. age group and genderbased distribution showed among male’s high isolation rate was observed in 21-40 years while in females 41-60-year age group. Every isolate was (100%) susceptible to linezolid, vancomycin and teicoplanin, cotrimoxazole also showed very low rate of drug resistance only 8.3%, while penicillin Doxycycline, Ciprofloxacin, Erythromycin showed 95.9%,69.8%55.4% and 48.2% drug resistance respectively, 43-45% drugresistant rate observed in case of Co-amoxiclave, Methicillin, Gentamicin, Fusidic Acid, Amikacin, and Clindamycin Conclusion: Linezolid, Vancomycin, and Teicoplanin are best therapeutic Choices against Staphylococcus aureus associated infections
