Degen, Annie

Grailly, Sabrina

Leloup, Robert

Poumay, Yves

Smekens, Michèle

The integrin α6β4 is polarized towards the basal side of basal keratinocytes and helps anchor these cells to the basement membrane components. We have found that cultured human epidermal keratinocytes, when detached from their culture substratum, as for grafting, using the enzyme dispase, rapidly internalize the basal membrane domains containing the integrin α6β4, while integrins of the very late antigen subtype remain on the cell surface. Detachment and incubation at 4°C prevent this internalization, as well as the contraction of the detached sheet area. Subsequent incubation at 37°C initializes this contraction and allows the basal integrin α6β4 to be internalized. We took advantage of this blockage to label upon detachment using immunogold techniques, the α6 subunit present on the basal cell surface; then we studied its internalization with the electron microscope. This internalization pathway differs from classical receptor-mediated endocytosis, and intermediate filaments might possibly play a role in this process. Interestingly, 1 h after their internalization from the basal membrane, a third of the gold particles labeling the α6 subunit was found between lateral membranes of basal cells, strongly suggesting that the integrin α6β4 can be partly recycled to the cell surface in these conditions.</p

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RESEARCH OUTPUTS / RÉSULTATS DE RECHERCHEAuthor(s) - Auteur(s) :Publication date - Date de publication :Permanent link - Permalien :Rights / License - Licence de droit d’auteur :Bibliothèque Universitaire Moretus PlantinInstitutional Repository - Research PortalDépôt Institutionnel - Portail de la Rechercheresearchportal.unamur.beSpecific internalization of basal membrane domains containing the integrin  6  4 indispase-detached cultured human keratinocytesPoumay, Yves; Smekens, Michèle; Grailly, Sabrina; Degen, Annie; Leloup, RobertPublished in:European journal of cell biologyPublication date:1993Document VersionPublisher's PDF, also known as Version of recordLink to publicationCitation for pulished version (HARVARD):Poumay, Y, Smekens, M, Grailly, S, Degen, A & Leloup, R 1993, 'Specific internalization of basal membranedomains containing the integrin  6  4 in dispase-detached cultured human keratinocytes', European journal ofcell biology, vol. 60, pp. 12-20.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            • Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            • You may not further distribute the material or use it for any profit-making activity or commercial gain            • You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Download date: 13. Nov. 2019

Specific internalization of basal membrane domains containing the integrin α6β4 in dispase-detached cultured human keratinocytes

https://pure.unamur.be/ws/files/12312381/Poumay1993_EJCB60_12.pdf

Specific internalization of basal membrane domains containing the integrin α6β4 in dispase-detached cultured human keratinocytes

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