\u3ci\u3eN\u3c/i\u3e\u3csup\u3e5\u3c/sup\u3e-Phosphonoacetyl-L-ornithine (PALO): A convenient synthesis and investigation of influence on regulation of amino acid biosynthetic genes in \u3ci\u3eSaccharomyces cerevisiae\u3c/i\u3e

Abstract

A scalable four-step synthesis of the ornithine transcarbamylase inhibitor N5-phosphonoacetyl-L-ornithine (PALO) is achieved through boroxazolidinone protection of ornithine. Investigations in the model organism Saccharomyces cerevisiae found that, in contrast to a previous report, PALO did not influence growth rate or expression of genes involved in arginine metabolism