In this article we review the species-specific genetic markers in the blood that are currently being used to authenticate the bison ancestry of animals entering the Ancestry Registry of the American Beefalo World Registry.  Independently of that program we are also testing for these same genetic markers in a number of private herds in which attempts are being made to develop bison-cattle hybrids that are fully fertile and incorporate desirable traits of both ancestral species

Dodd, J.

Morris, B. G.

Stormont, C. J.

Suzuki, Y.

English

UNL | Libraries

University of Nebraska - Lincoln 
DigitalCommons@University of Nebraska - Lincoln 
3rd World Congress on Genetics Applied to 
Livestock Production Animal Science Department 
1986 
BLOOD TYPING BEEFALO CATTLE 
C. J. Stormont 
Stormont Laboratories, Inc. 
B. G. Morris 
Stormont Laboratories, Inc. 
Y. Suzuki 
Stormont Laboratories, Inc. 
J. Dodd 
Stormont Laboratories, Inc. 
Follow this and additional works at: https://digitalcommons.unl.edu/wcgalp 
 Part of the Animal Sciences Commons 
Stormont, C. J.; Morris, B. G.; Suzuki, Y.; and Dodd, J., "BLOOD TYPING BEEFALO CATTLE" (1986). 3rd 
World Congress on Genetics Applied to Livestock Production. 2. 
https://digitalcommons.unl.edu/wcgalp/2 
This Article is brought to you for free and open access by the Animal Science Department at 
DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in 3rd World Congress on 
Genetics Applied to Livestock Production by an authorized administrator of DigitalCommons@University of Nebraska 
- Lincoln. 
BLOOD nPIRG BEEFALO CATTLE 
• c. J. STORMONT, B. G. MORRIS, Y. SUZUKI and J. DODD 
U. S. A. 
SUMMARY 
i le we review the species-specific genetic markers in the blood 
D this ar\~y being used to authenticate the bison ancestry of animals 
at' curre~ estry Registry of the American Beefalo World Registry. 
at iii the of ntchat program we are also testing for these same genetic markers 
.ntlY ivate herds in which attempts are being made to develop bison-
• _bel' of ~~at are fully fertile and incorporate desirable traits of both 
• b1brids 
u species. 
IRTRODOCTIOH 
11 the advent of the Basalo Hybrid Beefalo (BHB) in the early 1970s, 
Dt little more than academic interest in genetic markers in the blood 
::~t be used to distinguish between bison, cattle and their hybrids. BHB 
ere said to be 3/8 American buffalo (Bison bison or .llQ§. bison) and 5/8 :lCv(~~). Despite the world-wide publicity which this new breed 
.~, there was no published i~formation 00n its oderivatoion. This, coupled 
til. observation that these an~mals exhib~ted l~ttle, ~f any, superficial 
ance to bison, raised questions concerning their genealogy, especially in 
.ntific community. 
InDtually we obtained blood samples from 7 fullblood and 141 halfblood 
TIle blood samples were tested for the presence or absence of a number of 
cific genetic markers detected by means of starch-gel electrophoresis, 
, bison-specific hemoglobin (Hb), transferrin (Tf) and two electrophoretic 
of the red blood cell (RBC) enzyme known as carbonic anhydrase (CA). We 
toted the RBCs of these animals with anti body reagents which react wi th one 
... lpecies-specific antigenic markers on bison RBCs • 
• eetimated the percentage of animals of bison fractions ranging from 7/8 
16 that would be expec'ted to have at least one of these bison-specific 
e aarkers in their blood. These estimates, shown in Table 1, were on the 
the side for two reasons. We used a combined frequency of 0.84 for the 
alleles that code for the bison-specific forms of CA, namely, CA1 and CA2, 
~w know that the combined frequencies of those two alleles is closer to 
Por convenience in making those estimates, we assumed that our bison-
e antibody reagents were reacting with one species-specific antigen when 
Ut.ly that these reagents react with more than one bison-specific antigen. "b~own in Table 2, we found none of these bison-specific genetic markers 
IBC ood of the 7 animals represented to us as full blood BHBs and only one 
antigenic marker) in 141 animals represented to us as halfblood BHBs. 
rllults were reported in a brief note (Stormont, Morris and Suzuki) I 
ODt Laboratories, Inc., 1237 E. Beamer St., Suite D, Woodland, CA 95695 
published in 1977. 
Subsequent to these initial studies the World Beefalo Associati 
and not long thereafter a splinter organization, known as the oQ 
Beefalo Breeders Registry, came int~ existence. In the course of 
typing services for these two organ~zations, we put on record th 
virtually all the foundation BHBs plus additional purebred BH~ 
these two registries, a total of 46 bulls and 54 cows. None of 
possessed any of the aforementioned genetic markers. Many were 
electrophoretic forms of the RBC enzyme known as 6-pnosplnolglllC()~ltA 
(PGD) but none of them possessed the bison-specific form of that 
amplifying the conclusion that they probably had no bison ancestor 
Analysis of phenogroups in the B system of cattle blood grOUS; 
most definitive way of distinguishing between breeds of cattle P 
1984). Analysis of the B phenogroups in full blood BHBs indicated 
was probably derived by breeding Charolais, Angus and Hereford 
with a preponderance of Charolais breeding. We cannot, however 
possibility some Holsteins and Shorthorns may have been used in the 
of the BHB. 
Let us now turn to a brief review of the species-specific 6gual.~G 
the blood and the basis for their use in distinguishing between 
their hybrids. 
THE SPECIES-SPECIFIC GE1IETIC HAHXERS 
Those Defined by Serologic Tests. 
One of the classical ways of distinguishing between two 
species is to prepare antibody reagents which will react with 
agglutinate or hemolyse in the presence of complement) the RBC. 
species but not the other, and the converse. 
This can often be done by preparing an antiserum in domestic 
the one species (the homologous species) and absorbing all the an'~~~~ 
antiserum that will react with the RBCs of the other species (the 
species). When this is done, it is generally the rule that such 
still contain some antibodies specific for the RBCs of the 
But sometimes we are not always successful in producing these 
antibody reagents. Such was the experience of Owen, Stormont and 
the initial study of blood groups in American buffalo. 
We have been successful in producing bison-specific antibody 
only using antisera produced in rabbits but also antisera produced 
immunizing with bison RBCs. On the other hand, all of our ca 
reagents have been prepared by using bison RBCs 'to absorb antisera 
rabbits. 
Positive reactions with the bison-specific antibody reagents 
by the letter B whereas positive reactions with the catt~EI-spelo~1 
indicated by the letter C. In testing bison-cattle hybrids with 
three types are possible, namely, B, BC and C. Any animal of 
authentic hybrid, whereas any animal of type B qualifies along with 
BC as having at least one bison-specific genetic marker. 
Those Defined by Electrophoretic Tests. 
The Hemoglobins. Braend and Stormont (1963) reported that all 
blood samples, obtained from two non-interbreeding populations, 
two-zoned pattern in which the leading zone migrated to a 
.' Hb tYle~ir:d the fast zone (B) of cattle Hb whereas the trailing or slow 
tton just the same position as the slow zone of cattle Hb, namely, zone A • 
• urated to rphi c two-zoned pattern of bison Hb is clearly distinct from monomo , Uds Hb types, A, AB and B, found in the taurine breeds of cattle. 
~. coJDIDon ymbol bi to indicate the presence of bison Hb. The possible 
• use the Sntered in the hybrids are bi, Abi, biB, A, Band AB. Any animal 
of Hb enc~~B is an authentic hybrid, whereas any animal of type bi or Abi 
_,p' ,bi or t one bison-specific genetic marker. ;OS bI" at leas 
• _ .~Ansf~' In the aforementioned report, Braend and stormont pointed 
~3 bison were of the same Tf type, a three-zone pattern which, 
u.t all d ' tions of their runs, appeared to be indistinguishable from the 
til' conttern of Tf type A of domestic cattle. 
IOn' pa tem of buffers which were more effective in revealing differences 
OalDi a s~:ormont (1964) was able to distinguish the bison Tf type from all 
ft t,pes, coded for by the common Tf alleles (TFA, TfD1, TfD2 and TfE) of 
&II' Tt tYPdes thereby adding another bison-specific genetic marker which could 
bree s, . 
III for in the hybr~ds. . . . 
_ted ani 1 of Tf phenotype Abi or biD1 or biD2 (or biD where the d~stinchon 
iII1 ::d D may not be clear) or biE are authentic hybrids, whereas any 
, .. n:s~essini anyone of those types, or bi Tf alone, qualifies as having at 
0111 bison-specifiC genetic marker. 
De Carbonic Anhydrases. Sartore, Stormont, Morris and Grunder (1969) 
b:d two electrophoretic forms of CA in domestic cattle and three in bison. 
nae alleles for the two forms of cattle CA were designated CAF and CAS and 
.. produce phenoty:pes F, FS and S. The alleles for the three forms of bison 
d.lligna ted CA 1, CA 2 and CA 3 and these produce phenotypes 1, 1-2, 1-3, 2, 
) ud 3. When the bison CA types were compared with those of cattle it was 
'eDt tllat the zones coded for by the bison alleles CA 1 and CA2 occupied 
Uooa which were clearly ahead of the fast zone (F) of cattle. In contrast, 
3 of bison occupied an electrophoretic posi tion which appeared to be the 
'II tllat occupied by the slow zone (S) of cattle. Thus, only the zones CA1 
W of bison could be utilized as bison-specific genetic markers. 
IJI the various bison-cattle hybrids any of the six bison CA phenotypes and 
~ the three cattle CA phenotypes are possible. However, since zone CA-3 of 
11. indistinguishable from CA-S of cattle, we use only the symbol S in 
UII8 those phenotypes in which either 3 of bison or S of cattle could be 
t. 
!be distinguishable phenotypes in the hybrids are 1, 1-2, 1-F, 1-S, 2, 2-F, 
" FS and S. Any animal of phenotype 1-F or 2-F is an authentic hybrid 
I In animal of any of the following phenotypes has at least one bison-
C pnetic marker : 1, 1-2, 1-F, 1-S, 2, 2-F and 2-S. 
n. 6-Phosohogluconate Dehydrogenases. Contrary to results published by 
IDd Anderson (1970), Suzuki, Auditore, Morris and Stormont (1979) observed 
UN enzyme 6-phosphogluconate dehydrogenase is monomorphic in bison, just as 
l11n clttle, with the bison zone (labelled B) migrating ahead of the cattle 
(labelled C), thereby providing another genetic marker which can be used to 
ab between the two speCies and their hybrids. 
II the hybrids three phenotypes (B, BC and C) are possible. Any animal of 
tJpe Be is an authentic hybrid whereas any animal of phenotype B or BC 
6PG~ It least one bison-specific genetiC marker. 
l_U1s a homo-dimer. That is to say, each molecule of 6PGD is composed of 
1. ~ POlypeptide chains. In hybrids of phenotype BC we see not only the 
er c zones, Band C, but also see intermediate between those zones a 
------- ....... 
------"='-~-=-~- - ------
hybrid zone. The hybrid zone is a hetero-dimer in which ea 
composed of one B polypeptide chain and one C polypeptide chain. ch 
Other Markers. In addition to tests for the aforementioned 
electrophorese the blood plasma of all hybrids in polyacrylamide 
method described by Gahne, Juneja and Gromulus (1977) in attempts 
between bison, cattle and their hybrids by analysis of the Gc (vi 
protein) and post-transferrin-2 (PTf-2) zones. The reason we do 
J. Kraay, in a poster demonstration presented at the 19th 
Conference on Animal Blood Groups and Biochemical Genetics 
July, 1984, presented line drawings showing distinct ' 
and cattle with respect to these two systems of proteins. At 
when the blood samples are very fresh, we can see real differences 
PTf-2 zones of cattle and that of bison but we have not been able t 
distinction between the Gc zones of the two species. In 0 
polyacrylamide gels we have found that the PTf-2 zones of the hy 
reliably distinguished from those of the parental species. 
In a study of 19 systems of isozymes in the tissues (kidne 
muscle) of a variety of mammalian species, including ~ ~ an:' 
Baccus, Ryman, Smith, Reuterwall and Cameron (1983) reported 
cattle were distinctiy different with respect to only one enzyme 
which is consistent with the earlier observations of Suzuki .&t. ~ ( 
also recorded two forms of glutamate oxalate transaminase (GOT-1) 18 
of which was the same as that observed in cattle. But the numbers 
studied (7 bison and 6 cattle) were hardly sufficient to decide 
species differ at the GOT-1 locus. 
DISCUSSIOB 
Whether anything can be gained by searching for additional 
genetic markers in the blood that might be used to distinguish 
cattle and their hybrids is debatable. We take the position 
provided by our laboratory are more than ' adequate for the 
animals being considered for registration in the Ancestry 
American Beefalo World Registry. ABWR has no requirement for 
genetic markers in animals less than 3/8 bison. For animals 3/8 
not including 1/2 bison, one or more bison-specific markers is 
bison up to 3/4 bison, two or more bison-specific markers are 
animals over 3/4 bison, three or more bison-specific markers are 
published in Vol. 2, No.4 (July 26, 1985) of the Beefalo ~. 
by the data in Table 1, there is virtually no chance that an a 
truly 3/8 bison, or more, would fail to exhibit at least one of the 
bison-specific markers upon which the data in that table are based. 
After our experiences in testing BHBs for bison-specific 
is, of course, gratifying to find that there are now many hy 
do possess bison-specific genetic markers and that there is a 
represent those animals. The Officers, Board of Directors and 
organization realize that if the Beefalo is to gain credibility 1 
animal scientists and the general public it must exhibit some genes 
bison ancestors. 
R 
- -_/ 
REFERENCES 
N., SMITH, M. H., REUTERWALL, C. and CAMERON, D. 1983. 
and differentiation of large grazing mammals. J. Mamm. 64, 
d STORMONT, c. 1963 • . Haemoglobin and transferrin types in A~erican 
D, M.~ 197, 910-91,. 
;;,tal0' JA K and GROMULUS, J. 1977. Horizontal polyacrylamide gradient 
, &, ~~or~si~ for the simUltaneous phenotyping of tranferrin, post-
tl.ctrop albumin and post-albumin in the blood plasma of cattle. 
~;ps biochem. Genet. 8, 127-137. 
nd ANDERSON, D. c. 1970. Study of glucose 6-phosphogluconate 
I, S. N. a nd 6_phOSphogluconate dehydrogenase in American buffalo (~ geoase a 
• ~chem. Genet. 4, 651. . 
STORMONT, C. and IRW IN, M. R. 1958. Studies on blood groups in the 
II, J. D"rralo (bison) . Eyolution 12, 102-110 • 
..,-lean bu 
G STORMONT, C., MORRIS, B. G. and GRUNDER, A. A. 1969. Multiple 
.~ r~tic forms of carbonic anhydrase in red cells of domestic cattle ~ 
trO .~d Ameri can buffalo (Bison bison). Genetics 61, 823-831. 
comparison of bison and cattle transferrins. 
Band C systems of cattle revisited. Pp 31-43 in 
Ed. by W. H. Hildemann. Elsevier/North Holland. 
1984. Genetic markers in the blood and their application in animal 
319-327 in Proceedings of the 2nd World Congress on Sheep and Beef 
South African Stud Book and Livestock Improvement Association. 
INO T, C. J., MORR IS, B. and SUZUKI, Y. 1977. Analysis of blood groups in 
bison-cattle hybrids. Anim. Blood Grps biochem. Genet. 8, 
II, I., AUDITORE, K. J., MORRIS, B. and STORMONT, c. 1979. Transient 
rophoretic forms of red cell 6PGD in American buffalo (~.Qil.Qn) and 
Uc cattle (l&§. taurus). Biochem. Genet. 17, 445-448. 
Table 1. Percentage of animal s expected to 
bison-specific markers, namely, the 
Tf and CA. 
Table 2. 
Bison fraction 
7/8 
3/4 
1/2 
31/64 
7/16 
3/8 
5/16 
1/4 
3/16 
1/8 
1/16 
· ................................. . 
· ................................. . 
· ................................. . 
- (0.031253 x 0.18625) .......... 
- (0.1253 x 0.265) .............. 
- (0.253 x 0.37) •••••••••••••••• 
- (0.3753 x 0.475) •••••••••••••• 
- (0.53 x 0.58) ••••••••••••••••• 
- (0.6253 x 0.685) •••••••••••••• 
- (0.753 x 0.79) ................ 
- (0.8753 x 0.895) ••••••••••• • •• 
Comparison of observed and expected numbers of 
various bison fractions for the presence of at least one 
four bison-specific markers. 
~llin. No. testeg NQ. H;i.tb Slj; J,easj; Qne marker 
7/8 2 2 
3/4 6 6 
1/2 7 7 
31/64 
7/16 
7/16- 1 0 
3/8 4 2 
3/8- 7 0 
5/16 3 0 
1/4 12 9 
3/16- 141 
1/8 9 3 
1/16 2 
-
Basolo Hybrid Beefalo (BHB) Breeding. 
Z 


BLOOD TYPING BEEFALO CATTLE

DigitalCommons@University of Nebraska

University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln 3rd World Congress on Genetics Applied to Livestock Production Animal Science Department 1986 BLOOD TYPING BEEFALO CATTLE C. J. Stormont Stormont Laboratories, Inc. B. G. Morris Stormont Laboratories, Inc. Y. Suzuki Stormont Laboratories, Inc. J. Dodd Stormont Laboratories, Inc. Follow this and additional works at: https://digitalcommons.unl.edu/wcgalp  Part of the Animal Sciences Commons Stormont, C. J.; Morris, B. G.; Suzuki, Y.; and Dodd, J., "BLOOD TYPING BEEFALO CATTLE" (1986). 3rd World Congress on Genetics Applied to Livestock Production. 2. https://digitalcommons.unl.edu/wcgalp/2 This Article is brought to you for free and open access by the Animal Science Department at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in 3rd World Congress on Genetics Applied to Livestock Production by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. BLOOD nPIRG BEEFALO CATTLE • c. J. STORMONT, B. G. MORRIS, Y. SUZUKI and J. DODD U. S. A. SUMMARY i le we review the species-specific genetic markers in the blood D this ar\~y being used to authenticate the bison ancestry of animals at' curre~ estry Registry of the American Beefalo World Registry. at iii the of ntchat program we are also testing for these same genetic markers .ntlY ivate herds in which attempts are being made to develop bison-• _bel' of ~~at are fully fertile and incorporate desirable traits of both • b1brids u species. IRTRODOCTIOH 11 the advent of the Basalo Hybrid Beefalo (BHB) in the early 1970s, Dt little more than academic interest in genetic markers in the blood ::~t be used to distinguish between bison, cattle and their hybrids. BHB ere said to be 3/8 American buffalo (Bison bison or .llQ§. bison) and 5/8 :lCv(~~). Despite the world-wide publicity which this new breed .~, there was no published i~formation 00n its oderivatoion. This, coupled til. observation that these an~mals exhib~ted l~ttle, ~f any, superficial ance to bison, raised questions concerning their genealogy, especially in .ntific community. InDtually we obtained blood samples from 7 fullblood and 141 halfblood TIle blood samples were tested for the presence or absence of a number of cific genetic markers detected by means of starch-gel electrophoresis, , bison-specific hemoglobin (Hb), transferrin (Tf) and two electrophoretic of the red blood cell (RBC) enzyme known as carbonic anhydrase (CA). We toted the RBCs of these animals with anti body reagents which react wi th one ... lpecies-specific antigenic markers on bison RBCs • • eetimated the percentage of animals of bison fractions ranging from 7/8 16 that would be expec'ted to have at least one of these bison-specific e aarkers in their blood. These estimates, shown in Table 1, were on the the side for two reasons. We used a combined frequency of 0.84 for the alleles that code for the bison-specific forms of CA, namely, CA1 and CA2, ~w know that the combined frequencies of those two alleles is closer to Por convenience in making those estimates, we assumed that our bison-e antibody reagents were reacting with one species-specific antigen when Ut.ly that these reagents react with more than one bison-specific antigen. "b~own in Table 2, we found none of these bison-specific genetic markers IBC ood of the 7 animals represented to us as full blood BHBs and only one antigenic marker) in 141 animals represented to us as halfblood BHBs. rllults were reported in a brief note (Stormont, Morris and Suzuki) I ODt Laboratories, Inc., 1237 E. Beamer St., Suite D, Woodland, CA 95695 published in 1977. Subsequent to these initial studies the World Beefalo Associati and not long thereafter a splinter organization, known as the oQ Beefalo Breeders Registry, came int~ existence. In the course of typing services for these two organ~zations, we put on record th virtually all the foundation BHBs plus additional purebred BH~ these two registries, a total of 46 bulls and 54 cows. None of possessed any of the aforementioned genetic markers. Many were electrophoretic forms of the RBC enzyme known as 6-pnosplnolglllC()~ltA (PGD) but none of them possessed the bison-specific form of that amplifying the conclusion that they probably had no bison ancestor Analysis of phenogroups in the B system of cattle blood grOUS; most definitive way of distinguishing between breeds of cattle P 1984). Analysis of the B phenogroups in full blood BHBs indicated was probably derived by breeding Charolais, Angus and Hereford with a preponderance of Charolais breeding. We cannot, however possibility some Holsteins and Shorthorns may have been used in the of the BHB. Let us now turn to a brief review of the species-specific 6gual.~G the blood and the basis for their use in distinguishing between their hybrids. THE SPECIES-SPECIFIC GE1IETIC HAHXERS Those Defined by Serologic Tests. One of the classical ways of distinguishing between two species is to prepare antibody reagents which will react with agglutinate or hemolyse in the presence of complement) the RBC. species but not the other, and the converse. This can often be done by preparing an antiserum in domestic the one species (the homologous species) and absorbing all the an'~~~~ antiserum that will react with the RBCs of the other species (the species). When this is done, it is generally the rule that such still contain some antibodies specific for the RBCs of the But sometimes we are not always successful in producing these antibody reagents. Such was the experience of Owen, Stormont and the initial study of blood groups in American buffalo. We have been successful in producing bison-specific antibody only using antisera produced in rabbits but also antisera produced immunizing with bison RBCs. On the other hand, all of our ca reagents have been prepared by using bison RBCs 'to absorb antisera rabbits. Positive reactions with the bison-specific antibody reagents by the letter B whereas positive reactions with the catt~EI-spelo~1 indicated by the letter C. In testing bison-cattle hybrids with three types are possible, namely, B, BC and C. Any animal of authentic hybrid, whereas any animal of type B qualifies along with BC as having at least one bison-specific genetic marker. Those Defined by Electrophoretic Tests. The Hemoglobins. Braend and Stormont (1963) reported that all blood samples, obtained from two non-interbreeding populations, two-zoned pattern in which the leading zone migrated to a .' Hb tYle~ir:d the fast zone (B) of cattle Hb whereas the trailing or slow tton just the same position as the slow zone of cattle Hb, namely, zone A • • urated to rphi c two-zoned pattern of bison Hb is clearly distinct from monomo , Uds Hb types, A, AB and B, found in the taurine breeds of cattle. ~. coJDIDon ymbol bi to indicate the presence of bison Hb. The possible • use the Sntered in the hybrids are bi, Abi, biB, A, Band AB. Any animal of Hb enc~~B is an authentic hybrid, whereas any animal of type bi or Abi _,p' ,bi or t one bison-specific genetic marker. ;OS bI" at leas • _ .~Ansf~' In the aforementioned report, Braend and stormont pointed ~3 bison were of the same Tf type, a three-zone pattern which, u.t all d ' tions of their runs, appeared to be indistinguishable from the til' conttern of Tf type A of domestic cattle. IOn' pa tem of buffers which were more effective in revealing differences OalDi a s~:ormont (1964) was able to distinguish the bison Tf type from all ft t,pes, coded for by the common Tf alleles (TFA, TfD1, TfD2 and TfE) of &II' Tt tYPdes thereby adding another bison-specific genetic marker which could bree s, . III for in the hybr~ds. . . . _ted ani 1 of Tf phenotype Abi or biD1 or biD2 (or biD where the d~stinchon iII1 ::d D may not be clear) or biE are authentic hybrids, whereas any , .. n:s~essini anyone of those types, or bi Tf alone, qualifies as having at 0111 bison-specifiC genetic marker. De Carbonic Anhydrases. Sartore, Stormont, Morris and Grunder (1969) b:d two electrophoretic forms of CA in domestic cattle and three in bison. nae alleles for the two forms of cattle CA were designated CAF and CAS and .. produce phenoty:pes F, FS and S. The alleles for the three forms of bison d.lligna ted CA 1, CA 2 and CA 3 and these produce phenotypes 1, 1-2, 1-3, 2, ) ud 3. When the bison CA types were compared with those of cattle it was 'eDt tllat the zones coded for by the bison alleles CA 1 and CA2 occupied Uooa which were clearly ahead of the fast zone (F) of cattle. In contrast, 3 of bison occupied an electrophoretic posi tion which appeared to be the 'II tllat occupied by the slow zone (S) of cattle. Thus, only the zones CA1 W of bison could be utilized as bison-specific genetic markers. IJI the various bison-cattle hybrids any of the six bison CA phenotypes and ~ the three cattle CA phenotypes are possible. However, since zone CA-3 of 11. indistinguishable from CA-S of cattle, we use only the symbol S in UII8 those phenotypes in which either 3 of bison or S of cattle could be t. !be distinguishable phenotypes in the hybrids are 1, 1-2, 1-F, 1-S, 2, 2-F, " FS and S. Any animal of phenotype 1-F or 2-F is an authentic hybrid I In animal of any of the following phenotypes has at least one bison-C pnetic marker : 1, 1-2, 1-F, 1-S, 2, 2-F and 2-S. n. 6-Phosohogluconate Dehydrogenases. Contrary to results published by IDd Anderson (1970), Suzuki, Auditore, Morris and Stormont (1979) observed UN enzyme 6-phosphogluconate dehydrogenase is monomorphic in bison, just as l11n clttle, with the bison zone (labelled B) migrating ahead of the cattle (labelled C), thereby providing another genetic marker which can be used to ab between the two speCies and their hybrids. II the hybrids three phenotypes (B, BC and C) are possible. Any animal of tJpe Be is an authentic hybrid whereas any animal of phenotype B or BC 6PG~ It least one bison-specific genetiC marker. l_U1s a homo-dimer. That is to say, each molecule of 6PGD is composed of 1. ~ POlypeptide chains. In hybrids of phenotype BC we see not only the er c zones, Band C, but also see intermediate between those zones a ------- ....... ------"='-~-=-~- - ------hybrid zone. The hybrid zone is a hetero-dimer in which ea composed of one B polypeptide chain and one C polypeptide chain. ch Other Markers. In addition to tests for the aforementioned electrophorese the blood plasma of all hybrids in polyacrylamide method described by Gahne, Juneja and Gromulus (1977) in attempts between bison, cattle and their hybrids by analysis of the Gc (vi protein) and post-transferrin-2 (PTf-2) zones. The reason we do J. Kraay, in a poster demonstration presented at the 19th Conference on Animal Blood Groups and Biochemical Genetics July, 1984, presented line drawings showing distinct ' and cattle with respect to these two systems of proteins. At when the blood samples are very fresh, we can see real differences PTf-2 zones of cattle and that of bison but we have not been able t distinction between the Gc zones of the two species. In 0 polyacrylamide gels we have found that the PTf-2 zones of the hy reliably distinguished from those of the parental species. In a study of 19 systems of isozymes in the tissues (kidne muscle) of a variety of mammalian species, including ~ ~ an:' Baccus, Ryman, Smith, Reuterwall and Cameron (1983) reported cattle were distinctiy different with respect to only one enzyme which is consistent with the earlier observations of Suzuki .&t. ~ ( also recorded two forms of glutamate oxalate transaminase (GOT-1) 18 of which was the same as that observed in cattle. But the numbers studied (7 bison and 6 cattle) were hardly sufficient to decide species differ at the GOT-1 locus. DISCUSSIOB Whether anything can be gained by searching for additional genetic markers in the blood that might be used to distinguish cattle and their hybrids is debatable. We take the position provided by our laboratory are more than ' adequate for the animals being considered for registration in the Ancestry American Beefalo World Registry. ABWR has no requirement for genetic markers in animals less than 3/8 bison. For animals 3/8 not including 1/2 bison, one or more bison-specific markers is bison up to 3/4 bison, two or more bison-specific markers are animals over 3/4 bison, three or more bison-specific markers are published in Vol. 2, No.4 (July 26, 1985) of the Beefalo ~. by the data in Table 1, there is virtually no chance that an a truly 3/8 bison, or more, would fail to exhibit at least one of the bison-specific markers upon which the data in that table are based. After our experiences in testing BHBs for bison-specific is, of course, gratifying to find that there are now many hy do possess bison-specific genetic markers and that there is a represent those animals. The Officers, Board of Directors and organization realize that if the Beefalo is to gain credibility 1 animal scientists and the general public it must exhibit some genes bison ancestors. R - -_/ REFERENCES N., SMITH, M. H., REUTERWALL, C. and CAMERON, D. 1983. and differentiation of large grazing mammals. J. Mamm. 64, d STORMONT, c. 1963 • . Haemoglobin and transferrin types in A~erican D, M.~ 197, 910-91,. ;;,tal0' JA K and GROMULUS, J. 1977. Horizontal polyacrylamide gradient , &, ~~or~si~ for the simUltaneous phenotyping of tranferrin, post-tl.ctrop albumin and post-albumin in the blood plasma of cattle. ~;ps biochem. Genet. 8, 127-137. nd ANDERSON, D. c. 1970. Study of glucose 6-phosphogluconate I, S. N. a nd 6_phOSphogluconate dehydrogenase in American buffalo (~ geoase a • ~chem. Genet. 4, 651. . STORMONT, C. and IRW IN, M. R. 1958. Studies on blood groups in the II, J. D"rralo (bison) . Eyolution 12, 102-110 • ..,-lean bu G STORMONT, C., MORRIS, B. G. and GRUNDER, A. A. 1969. Multiple .~ r~tic forms of carbonic anhydrase in red cells of domestic cattle ~ trO .~d Ameri can buffalo (Bison bison). Genetics 61, 823-831. comparison of bison and cattle transferrins. Band C systems of cattle revisited. Pp 31-43 in Ed. by W. H. Hildemann. Elsevier/North Holland. 1984. Genetic markers in the blood and their application in animal 319-327 in Proceedings of the 2nd World Congress on Sheep and Beef South African Stud Book and Livestock Improvement Association. INO T, C. J., MORR IS, B. and SUZUKI, Y. 1977. Analysis of blood groups in bison-cattle hybrids. Anim. Blood Grps biochem. Genet. 8, II, I., AUDITORE, K. J., MORRIS, B. and STORMONT, c. 1979. Transient rophoretic forms of red cell 6PGD in American buffalo (~.Qil.Qn) and Uc cattle (l&§. taurus). Biochem. Genet. 17, 445-448. Table 1. Percentage of animal s expected to bison-specific markers, namely, the Tf and CA. Table 2. Bison fraction 7/8 3/4 1/2 31/64 7/16 3/8 5/16 1/4 3/16 1/8 1/16 · ................................. . · ................................. . · ................................. . - (0.031253 x 0.18625) .......... - (0.1253 x 0.265) .............. - (0.253 x 0.37) •••••••••••••••• - (0.3753 x 0.475) •••••••••••••• - (0.53 x 0.58) ••••••••••••••••• - (0.6253 x 0.685) •••••••••••••• - (0.753 x 0.79) ................ - (0.8753 x 0.895) ••••••••••• • •• Comparison of observed and expected numbers of various bison fractions for the presence of at least one four bison-specific markers. ~llin. No. testeg NQ. H;i.tb Slj; J,easj; Qne marker 7/8 2 2 3/4 6 6 1/2 7 7 31/64 7/16 7/16- 1 0 3/8 4 2 3/8- 7 0 5/16 3 0 1/4 12 9 3/16- 141 1/8 9 3 1/16 2 -Basolo Hybrid Beefalo (BHB) Breeding. Z 

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BLOOD TYPING BEEFALO CATTLE

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