This study examines the spatial association between science- and technology-related entrepreneurship and neighbourhood walkability in eastern Nebraska, often referred to as the northern ‘Silicon Prairie’. Tech start-up firms are expected to gravitate toward more walkable or pedestrian-oriented urban locations to benefit from heightened social interaction, knowledge spillovers and a more vibrant, creative atmosphere. Data on start-up firms collected from the online database Crunchbase.com and a walkability index provided by the popular online service Walk Score® were used to evaluate the walkability–tech entrepreneurship nexus. The spatial relationship between walkability and firm location may have important implications for metropolitan areas seeking to incubate new firms and industries in situ, and potentially become leaders in new, innovative industries