The thermal stability of platinum based chemotherapeutic agents
Cisplatin, Oxaliplatin and Carboplatin interaction with 10 basepair DNA/RNA duplexes and hybrids was investigated. UV-Spectroscopy was used to measure the enthalpy, entropy, melting temperature, free energy, and binding stoichiometry of DNA/RNA duplexes and hybrids denaturation in the existence and the absence of Cisplatin, Oxaliplatin and Carboplatin. Isothermal Titration Calorimetry was used to measure the enthalpy, entropy, free energy, association/dissociation constants and stoichiometry of DNA/RNA duplexes and hybrids formation in the presence and the absence of Cisplatin, Oxaliplatin and Carboplatin
