People are more and more desire a youthful and attractive appearance. Aging of the skin can be the result of a normal process that involves a lower rate of metabolic activities and is called chronologic aging. Photoaging is the consequence of chronic sun exposure and manifests clinically with fine and coarse wrinkling, roughness, dryness,laxity, shallowness, pigmentary mottling, telangiectasia, and in some cases with preneoplastic and neoplastic changes (Stefanaki et al., 2005). Tretinoin is a compound that is able to show an effect of prevention and treatment of wrinkles due to photo aging, acne, and inflammation of the skin. It is all-trans retinol is oxidized through retinal to its most active metabolite (Kligman et. al, 1986). The effectivity of an active ingredient is determined among others by the ability to reach the site of action. To reach the site of action the active ingredients used orally or topically must first dissolve, release then absorbed or penetrates through the membrane and then diffuses into the site of action. Tretinoin practically insoluble to increase it used nanoemulsion delivery as a vehicle. Solubility is one of the factors of drug absorption. Higher solubility of drug caused higher amount of drug to absorb. Smaller droplet of emulsion caused rapid drug release. In this study tretinoin loaded in nanoemulsion using olive oil than it characterized and test of tretinoin release compared with tretinoin in emulsion. Olive oil used as oil phase in nanoemulsion as known it widely used in cosmetic
