Yasinska, O.V.

Цифровий Репозиторій - Інтелектуальні Фонди Буковинського державного медичного університету

51according to the Student's distribution tables were studied. Values for which p <0.05 wereconsidered probable.An average daily level of sodium ions in the urine increased reliably. High natrium uresiswas recorded at all study intervals. The acrophase remained unchanged, but the rhythm amplitudeincreased by 35%.Changes in the ion-regulatory function of the kidneys were also characterized bya probably high clearance of sodium ions during the observation period. Mesor was 0, b ± 0.15 ml /2 h and exceeded 500% of control animals. An average daily level of rhythm of proximal transportof sodium ions in all studied periods of the day was lower than in the control group of animals,which probably led to the elimination of excess of this cation from blood plasma. Distal transportalso decreased at all times of the day. The amplitude of the rhythm increased by 33%, and the mesordecreased by 78% compared with the control data. The phase structure of the rhythm did notchange. Summarizing the results of the study, it should be noted that in conditions of constantdarkness of the pineal gland changes in the chronostructure of the ion-regulating function of thekidneys are compensatory in nature. In particular, the probable decrease in the average daily levelof sodium ion reabsorption, the basal level of proximal and distal transport of sodium ions, whichleads to high natriuresis during the entire observation period, attracts attention.Tymofiychuk I.R.COGNITIVE DISABILITY IN ESTROGEN-ECTOMIZED AND OLD RATS WITHDEVELOPMENT OF DIABETES MELLITUSYa.D. Kirshenblat Department of PhysiologyBukovinian State Medical UniversityIn recent years, there have been many works dealing with the study of the effects of sexhormones on cognitive function.  Clinical studies have found that in menopausal women, thetendency to develop type 2 diabetes will increase, spatial and short-term working memory worsens,and there is a tendency to develop depression. The findings suggest that estrogens are involved inthe mechanisms of insulin resistance in tissues, in the synthesis of mediators in thecatecholaminergic systems of the brain, but many issues remain unresolved. The aim: to establishthe effect of estrogens on the indices of spatial memory in ovariectomized and old rats in thebackground of development of experimental diabetes.The study was conducted on 30 adult female rats 4-5 months and 20 months old. The studygroups were ovariectomized and experimental type 2 diabetes mellitus with protamine sulfate wassimulated. The study of spatial memory was carried out in an eight-sleeved radial labyrinth.Ovariectomy caused deterioration of spatial memory relative to the living control group, anddiabetes mellitus aggravated pathological changes.Our data allow us to draw conclusions about the influence of sex hormones on cognitivefunctions. Ovariectomy caused a deterioration of spatial memory, and the combination ofovariectomy and diabetes aggravated the pathological process. In older animals, the study found adecrease in spatial memory, and diabetes further aggravated the cognitive function. Changes thatoccur after ovariectomy indicate involvement of estrogen in the regulation of cognitive functions inthe process and indicate the possibility of using estrogens in the treatment of neurodegenerativechanges in premature and age-related menopause and in the background of concomitantpathological processes.Yasinska O.V.FEATURES OF THE PROTEIN METABOLISM IN THE ADRENAL TISSUES OFIMMATURE RATS WITH HYPOBARIC HYPOXIA DEPENDING ON SEX AND THEFUNCTIONAL ACTIVITY OF THE PINEAL GLANDYa.D. Kirshenblat Department of PhysiologyBukovinian State Medical UniversityHypoxia is one of the conditions of usual life style and, at the same time, a factor increasingreactive oxygen species (ROS) level. Structural-functional changes of the adrenal glands as52regulatory organs of adaptive process are a typical manifestation of organic reaction to hypobarichypoxia. A moderate intermittent hypoxia is used for altitude training to develop adaption at boththe systemic and cellular level.The nature of proteolytic activity in peripheral tissues changes in the process of response toa variety of environmental factors that may be both a manifestation of regenerative processes, andtheir involvement in the mechanisms of apoptosis, in particular, due to oxidative modification ofproteins.Aim of research is to investigate sex-related dependence of the reaction of proteinmetabolism in tissues of the adrenal glands of immature albino rats under conditions of systemicintermittent hypobaric hypoxia and altered photoperiod. Experiments were carried out on 56 maleand 60 female immature laboratory rats aged 1 month. Hypobaric hypoxia, equivalent to an altitudeof 4000 m above sea level, was used for 2 hours, for 14 days in the background of three lightingmodes: natural lighting, constant round-the-clock lighting and constant round-the-clock darkness.Proteolytic activity was determined according azoalbumin, azokazein and azokol lysis as indices oflow molecular weight protein lysis, high molecular weight protein lysis and collagen lysis. Thedegree of oxidative modification of proteins in the adrenal glands was assessed by the amount of2,4-dinitrophenylhydrazone of neutral and alkaline nature.A sex dependent difference in the activity of proteolytic processes and intensity of proteinperoxidation in the adrenal glands in immature rats was found. In intact male rats intensity ofproteolysis is significantly lower than in female rats. Modeling of a decreased melatonin-producingfunction of the pineal gland by application of constant lighting resulted in significant increase of theactivity of proteolytic processes in the tissues of the adrenal glands in both male and femaleimmature rats, that may be indicative of intensification in elimination of oxidation-modified proteinmolecules, formed by reducing of tissues antioxidant capacity according to melatonin deficiency.Simultaneous action of hypobaric hypoxia and permanent lighting caused the utmost increase ofintensity of proteolysis in the experimental groups, particularly in regard to macromolecularproteins. Simultaneous action of hypobaric hypoxia and permanent darkness caused a reverseresponse, which was manifested in normalization of proteolysis indices, decreased by hypoxia.These results can be indicative of the fact that constitutional sexual differences are pronouncedmore in case of an isolated action of the applied factors, while in case of considerable exertion ofadaptive mechanisms in combination of hypoxia with pineal gland dysfunction such kinds ofdifferences are leveled.The sex related dependence of response mechanisms of proteolytic processes in theirinteraction with the processes of peroxidation of proteins and their role in adaptive restructuring ofthe adrenal glands tissues under conditions of hypoxic preconditioning of the damaging effects ofthe modified duration of photoperiod by using of moderate hypobaric hypoxia require furtherinvestigation and comprehensive analysis.Анохіна С.І.ВПЛИВ ЕКЗОГЕННОГО МЕЛАТОНІНУ ЗА УМОВ ГІПО- ТА ГІПЕPТИPЕОЇДНОГОСТАНУ НА ЗМІНИ ПОКАЗНИКІВ ФІБPИНОЛІЗУ В ТКАНИНІ ПЕЧІНКИОСЛІПЛЕНИХ ЩУPІВКафедра фізіології ім. Я.Д.КіршенблатаБуковинський державний медичний університетШишкоподібна залоза або епіфіз - нейроендокринне утворення  яке володіє дужеширокими інтегративними властивостями через мелатонін, з одного боку модулюєнейроендокринні функції, з іншого – сама є об’єктом керування різноманітнимигормональними та гуморальними сигналами. Епіфіз – нейpоендокpинне утвоpення, якеспpияє тpансфоpмації сигналів зовнішнього сеpедовища в гумоpальні стимули і яке здатнеpегулювати функціювання гіпоталамо-гіпофізаpно-тиpеоїдного комплексу. Наявні деякіповідомлення пpо підвищення pівня мелатоніну у хвоpих із патологіями травної системи тасеpцево-судинними захвоpюваннями. Особливо небезпечне, поpушення збалансованості

Features of the protein metabolism in the adrenal tissues of immature rats with hypobaric hypoxia depending on sex and the functional activity of the pineal gland

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Features of the protein metabolism in the adrenal tissues of immature rats with hypobaric hypoxia depending on sex and the functional activity of the pineal gland

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Цифровий Репозиторій - Інтелектуальні Фонди Буковинського державного медичного університету