Douma, A.C.

Driessen, A.J.M.

Harder, W.

Veenhuis, M.

English

ARTS repository - University of Groningen

   University of GroningenLiposome-Mediated Introduction of Foreign Proteins into the Matrix of Protoplasts and into thePeroxisomal Membrane of the Methanol-Grown Yeast Hansenula polymorphaDouma, A.C.; Veenhuis, M.; Driessen, A.J.M.; Harder, W.IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1988Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Douma, A. C., Veenhuis, M., Driessen, A. J. M., & Harder, W. (1988). Liposome-Mediated Introduction ofForeign Proteins into the Matrix of Protoplasts and into the Peroxisomal Membrane of the Methanol-GrownYeast Hansenula polymorpha.CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Download date: 07-06-2022Abstracts o f  The Netherla,~ds Sot et)' o f  Electro. Microscopy 431 is made of clinical quality PMMA and is sutured episclerally with eight stain- less steel wires. Since PMMA is hydro- phobic, easily damaged and difficult to sterilize, we designed, developed and tested a new KP made of glass and fitted in a metal cylinder with flange of eith- er Inconel-600 or technically pure plat- inum. The anterior curvature of the glass was shaped by pressure-moulding or melting. Results: The optics of the melted glass core are superior to the moulded one and, moreover, contact between glass and metal was better. The Inconel-600 KP caused melting away and ulceration of the surrounding cornea, most likely due to metal ions. With the platinum~glass KP the only complication, if present, was corneal overgrowth. Conclusion: SEM images show that over a period of at least five months the platinum-glass KP did not show leakage, extrusion or adverse tissue reactions such as apocification, melting away, in- fection or epithelial downgrowth. From these data it would seem that a clinical trial of the presently available newly developed platinum-glass KP should be undertaken. MORPHOLOGY OF LINEAR-LOW-DENSITY POLYETHYLENE (LLDPE) R. Deblieck FA-OM, DSM Research, PO Box 18, NL 6160 MD Geleen The distribution of comonomer units in the chains of LLDPE is known to in- fluence the crystallization and melting behaviour. Typical Differential Scan- ning Calorimetry (DSC) curves feature a broad melting temperature range and, moreover, show two major peaks. It has been shown that the crystallite size distribution suggested by these curves is caused by intermolecular heterogeneity of the comonomer distri- bution. This intermoiecuiar hetero~- eity should thus be reflected in the lamellar morphology c f a LLDPE sample. Samples of Stamylex 1016 with sev- eral known thermal histories were fixed ~d stained by means of the usual HCISo 3 treatment in the vapour phase. Sections of about 60 nm were obtained at room temperature and stained with Uranyl acetate. Observation in CTEM clearly shows that the cooling rate affects the lam- ellar thickness and sizes: a distinct lamellar thickening is observed in sam- ples which have been isothermally crys- tallized below the highest melting peak. It is at this temperature that the chains with low comonomer content crystallize. These results were confirmed by means of a different fixation/staining method in bulk using RuO 4. LIPOSOME-MEDIATED INTRODUCTION OF FOREIGN PROTEINS INTO THE MATRIX OF PROTOPLASTS AND INTO THE PEROXISOMAL MEMBRANE OF THE METHANOL-GROWN YEAST HANSENULA POLYMORPHA A.C. Douma, M. Veenhuis*, A.J.M. Driessen and W. Harder Department of Microbiology and *Labor- atory for Electron Microscopy, Biolog- ical Centre, Kerklaan 30, 9751NN Haren Low-pH-induced fusion between lipo- somes and protoplasts or isolated perox- isomes of the methanol-grown yeast Han- senula polymorpha could be d~monstr~ted using a fluorescent assay and freez= etch techniques. Best results were 9b- tained with liposomes composed of car- diolipin and phosphatidylcholine. This method was used for the intro- duction of foreign proteins into proto- plasts and peroxisomes. After fusion or glucose oxidase containing liposomes with protoplasts, glucose oxidase activ- ity was demonstrated in the cytosol of the protoplasts with cytochemical tech- niques. Similarly ferritin could be in- troduced into the cytosol of protoplasts and also into the matrix of isolated peroxisomes, if ferritin was added to protoplasts together with empty lipo- somes, this protein was also introduced into the protoplasts. However, in the absence of liposomes no uptake of ferri- tin occurred. In addition, it was demonstrated that liposomes can be used as a carrier to introduce foreign proteins into the ~em- brane of isolated peroxisomes. After fusion of bacteriorhodopsin-containing liposomes with isolated peroxisomes freeze-etch experiments revealed the presence of large protein particles in the peroxisomal membrane which normally shows largely smooth fracture faces. These techniques are currently being used in the study of the biogenesis of yeast peroxisomes. 

Liposome-Mediated Introduction of Foreign Proteins into the Matrix of Protoplasts and into the Peroxisomal Membrane of the Methanol-Grown Yeast Hansenula polymorpha

https://pure.rug.nl/ws/files/98234050/1988UltramicroscDouma.pdf

Liposome-Mediated Introduction of Foreign Proteins into the Matrix of Protoplasts and into the Peroxisomal Membrane of the Methanol-Grown Yeast Hansenula polymorpha

Abstract

Similar works

Full text

Available Versions

ARTS repository - University of Groningen