A monitoring programme was carried out in Lombardy from 2000 to study the presence and
biology of the leafhoppers involved in phytoplasma transmission to grape. From 2003 also
biomolecular analyses were performed to assess the frequency of \u201cpositive\u201d insects using PCR.
Special attention was given to Hyalesthes obsoletus Signoret (Rhynchota: Cixiidae), a leafhopper
whose presence in vineyards is worrying because it is well known from literature that this insect can
transmit to the grape the \u201cbois noir\u201d, a phytoplasma disease whose symptoms are very similar to those
produced by the \u201cgolden flavescence\u201d. During the surveys this species was only occasionally collected
directly from grape, but it was quite abundant on weeds, mainly Urtica sp. and in a lesser extent,
Convolvulus arvensis and Calystegia sp.. The percentage of \u201cpositive\u201d specimens was quite different
in the various localities but on the average the percentage of positive ones was rather low. According
to these results, H. obsoletus does not seem to play a major role in transmitting the \u201cbois noir\u201d in
Lombardy, as the insect can be found only occasionally on grape, and moreover because the
percentage of \u201cpositive\u201d specimens, at least in some areas, is very low