Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, characterization of active ingredients in Capsicum frutescens fruits and Annona muricata L. leaves. Capsicum frutescens used for various problems with digestion including upset for conditions of the heart and blood vessels including poor circulation, excessive blood clotting, high cholesterol, and preventing heart disease. Whereas Annona muricata L. leaves are traditionally used to treat diabetes. People have been consuming raw leaves of Annona muricata L. to control blood glucose levels. The present investigation was designed to study the phytochemical profiling and bioactive component principles of Capsicum frutescens and Annona muricata by Thin layer Chromatography. Bio autography agar overlay test was done to detect the antimicrobial activity of the extracts. The results of this study confirmed the presence of various bioactive compounds in the acetone and methanol extracts of both the plants. The chromatographic analysis revealed that Capsicum frutescens fruit extract and Annona muricata leaf extract are composed of various Alkaloids, Terpenoids, Saponins, and Phenolics which are accountable for many biological activities. Bio autography assays shows that Capsicum frutescens shows growth inhibition against bacteria, but Annona muricata does not shown any significant activity. The findings of present study implies that both extracts are potent source for some medicinally important phytochemicals with antioxidant and antibacterial activities.
Keywords: Phytochemicals; Thin Layer Chromatography; Bio autography
