Development and validation of various spectrophotometric methods for naproxen as anti arthritis

Abstract

The present research work discusses the development of UV-spectrophotometric method for estimation of Naproxen. Simple, specific, accurate and cost effective spectrophotometric method has been developed for estimation of naproxen sodium in bulk as well as formulation. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λmax) was found to be 331 nm. The validation was performed as per ICH guidelines for linearity, accuracy, precision, LOD and LOQ. The linearity was found in the concentration range of 3-24 µg / ml for Zero, first and second order derivative spectroscopy. The correlation coefficients were found to be 0.9997, 0.9999 and 0.9998, respectively. The obtained (r2) values show that the selected concentration range gives good linearity. All calibration curves show a linear relationship between the absorbance and concentration with coefficient of correlation 0.999. The regression of curve was Y = 0.0372 x - 0.0038. The precision of method was found to be good. The results were within the range of 99.15 ± 0.14 – 101.38 ± 0.17 and were found to be highly accurate. The proposed method will be suitable for analysis of naproxen in bulk as well as pharmaceutical formulations in quality control purpose. It is thus concluded that the proposed method is new, simple, cost-effective, safe, accurate, precise and environmental friendly Keywords: Naproxen, Spectrophotometry, Naproxen, Linearity, Validation, LO