Broad genetic variability of starting biological material is crucial prerequisite for the successful breeding program. Maize Research Institute, owning Gene bank with more than 6,000 accessions consisted of the local and introduced collection, has the opportunity for the investigation both fundamental genetic processes, pre-breeding and breeding for commercial purposes. To search for new sources of maize drought tolerance Gene bank accessions were scored visually on stay-green phenotype and total appearance. More than 50 genotypes have been identified as a potential source for drought tolerance. Besides breeding on improved storage protein quality decreasing of phytate content in kernel is also under the consideration, because the most phosphorous is bound in phytate. In breeding program it is desirable to have genotypes with higher content of available phosphorous (P) not bound in phytate. Among 60 analyzed populations from Gene bank only one has been determined to have very low phytate content and will be used in maize breeding program on low phytate in kernel. Process of maize breeding, to get high yielding hybrids, begins by the genetic variability determination of starting breeding material, either populations or selected inbred lines. Genetic divergence of parental inbred lines is main step to get high heterotic effect in yield after crossing. Use of different molecular markers allowed cluster analysis by use of UPGMA methods and select high number of genotypes to be included in crossing process
