A Study of Methods for Detecting Gene Expression of the Sterol Biosynthetic Pathway in the Microalgae Strain Heterosigma akashiwo

Abstract

Biotechnological applications for various microalgae strains are beginning to bloom. There is interest in these microbes for their production of useful compounds such as phytosterols and lipids which can be produced in mass culture for large scale manufacture in the food, pharmaceutical, and biofuel industries (Romano et al., 2016). To this end, we are using the gold-brown microalgae, Heterosigma akashiwo (CCMP1680), a known producer of phytosterols, to test a molecular method for quick and easy measurements of production of the phytosterol compound sitosterol, which has potential as an anti- inflammatory agent. (Giner et al., 2008)

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