Multilayer-coated microneedles achieve transcutaneous delivery of plasmid DNA to the viable epidermis. Cy3-labeled plasmid DNA encoding luciferase (yellow) is deposited on biodegradable microneedle arrays through multilayer self-assembly and then delivered to the skin by microneedle application to achieve colocalization with Langerhans dendritic cells (MHC II-GFP–green).United States. Dept. of Defense (DAAD19- 02-D-0002)United States. Dept. of Defense (W911NF-07-D-0004)Ragon Institute of MGH, MIT and HarvardNational Institutes of Health (U.S.) (NIH Biotechnology Training Program at MIT)Howard Hughes Medical Institute (Investigator
