Haandrikman, Alfred J.

Kok, Jan

Konings, Wil N.

Leenhouts, Kees J.

Mierau, Igor

Tan, Paris S.T.

Venema, Gerard

English

The gene specifying an endopeptidase of Lactococcus lactis, named pepO, was cloned from a genomic library of L. lactis subsp. cremoris P8-247 in lambdaEMBL3 and was subsequently sequenced. pepO is probably the last gene of an operon encoding the binding-protein-dependent oligopeptide transport system Of L. lactis. The inferred amino acid sequence of PepO showed that the lactococcal endopeptidase has a marked similarity to the mammalian neutral endopeptidase EC 3.4.24.11 (enkephalinase), whereas no obvious sequence similarity with any bacterial enzyme was found. By means of gene disruption, a pepO-negative mutant was constructed. Growth and acid production of the mutant strain in milk were not affected, indicating that the endopeptidase is not essential for growth of L. lactis in milk.</p

ARTS repository - University of Groningen

   University of GroningenCLONING AND SEQUENCING OF THE GENE FOR A LACTOCOCCAL ENDOPEPTIDASE,AN ENZYME WITH SEQUENCE SIMILARITY TO MAMMALIAN ENKEPHALINASEMierau, Igor; Tan, Paris S.T.; Haandrikman, Alfred J.; Kok, Jan; Leenhouts, Kees J.; Konings,Wil N.; Venema, GerardPublished in:Journal of BacteriologyIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1993Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Mierau, I., Tan, P. S. T., Haandrikman, A. J., Kok, J., Leenhouts, K. J., Konings, W. N., & Venema, G.(1993). CLONING AND SEQUENCING OF THE GENE FOR A LACTOCOCCAL ENDOPEPTIDASE, ANENZYME WITH SEQUENCE SIMILARITY TO MAMMALIAN ENKEPHALINASE. Journal of Bacteriology,175(7), 2087-2096.CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.AUTHORS' CORRECTIONCloning and Sequencing of the Gene for a Lactococcal Endopeptidase,an Enzyme with Sequence Similarity to Mammalian EnkephalinaseIGOR MIERAU, PARIS S. T. TAN, ALFRED J. HAANDRIKMAN, BALTHASAR MAYO, JAN KOK,KEES J. LEENHOUTS, WIL N. KONINGS, AND GERARD VENEMADepartment of Genetics and Department of Microbiology, Centerfor Biological Sciences,University of Groningen, Kerklaan 30, 9751 NN Haren, The NetherlandsVolume 175, no. 7, p. 2087: The article byline should read as given above. The present address of Balthasar Mayo isDepartemento de Biologica Functional, Facultade de Medicina, Julian Claseria, S/N 33006 Oviedo, Spain.3918 at university library on March 26, 2007 jb.asm.orgDownloaded from 

CLONING AND SEQUENCING OF THE GENE FOR A LACTOCOCCAL ENDOPEPTIDASE, AN ENZYME WITH SEQUENCE SIMILARITY TO MAMMALIAN ENKEPHALINASE

https://pure.rug.nl/ws/files/3305260/1993JBacteriolMierauCorr.pdf

CLONING AND SEQUENCING OF THE GENE FOR A LACTOCOCCAL ENDOPEPTIDASE, AN ENZYME WITH SEQUENCE SIMILARITY TO MAMMALIAN ENKEPHALINASE

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