Umi Barokah Irawati. 2006. THE EFFECT OF ASPARTAME TO THE SERUM
CREATININE LEVEL AND HISTOLOGIC STRUCTURE OF KIDNEY IN
MICE (Mus musculus L.) STRAIN SWISS. Departement of Biology. Faculty of
Mathematics and Natural Science. Sebelas Maret University. Surakarta.
The aims of this research were to know the effect of aspartame to the
serum creatinine level and histologic structure of kidney (glomerules and
proximal tubules).
This research was evaluated experimentally to male mice with average
weight of 20 gr. These mice were divided randomly in to 5 groups i.e.: control, 5
mg aspartame, 10 mg aspartame, 15 mg aspartame, and 20 mg aspartame. The
treatment was done for 28 days, before treatment blood had taken and measured
creatinine serum level. After treatment mice blood had taken again, killed and
sectioned kidney organ to make histologic preparation. Creatinine serum level,
wide average Bowman space glomeruli and lumen proximal tubules was analyzed
using analysis of varians (ANOVA) and continued Duncan Multiple Range Test
(DMRT) on 5% significans, time factor of creatinine serum level was analyzed
using T-test. Observation of kidney histologic structure (glomerules and proximal
tubules) were done to determine the kind and level of damage and analyzed
descriptively.
Creatinine serum level control normaly dosage variation aspartame
creatinine serum level increase up to ± 3.29 mg/dl. There were cantrol glomerules
and proximal tubules normaly. Dosage variation aspartame glomerules damage,
i.e.: cloudy swelling degeneration; karyorectic; picnotic; karyolitic; wide Bowman
space and hemolitic erytrocyte, proximal tubules damages were picnotic;
karyorectic; karyolitic; hydrophic degeneration; lumen narrowed and protein cast.
Key Word : aspartame, creatinine, glomerules, proximal tubule
