Department of Fundamental Natural Science, Okayama University of Science
Abstract
Tricholoma matsutake is one of the parasitic fungi which weakly grow not only on the artificial culture media, but on the field. The isolation of Tricholoma matsutake hyphae and the culture of the hyphae were investigated in a test tube. We have been able to isolate hyphae using explants from gills of unspread cap of Tricholoma matsutake fruiting bodys. For the isolation of hyphae, it was concluded that the best medium was Hamada medium, the next one was 1/10 conc. Hamada medium and the third one was Waxman medium. Hamada medium has been mainly used for the culture medium because the better growthes of hyphae (60 mm/90 day) were achieved. The growth (elongation) rates of the hyphae were scarcely regarded as significant by the various additions. While, the forme of the mycelia were regarded as significant by the additions of inorganic salts and metal ions
