THE EFFECT OF TiO2 NANOPARTICLES (DOPED OR NOT WITH Ag, ENCAPSULATED OR NOT IN LYPOSOMES), ON THE SPLEEN ULTRASTRUCTURE IN Mus musculus SPECIES, EXPOSED AT A STRESS FACTOR X-RAYS

Abstract

The present experiment was performed on young females of Mus musculus, 22-24 g each, intraperitoneal injected with a suspension of titanium dioxide (five injections of 0.5 ml each, one at two days, with 0.01% TiO2 or TiO2-Ag suspension). The TiO2 nanoparticles of anatase crystallization form, 10-20 nm size, wereconjugated or not with 1% Ag, encapsulated or not in lyposome. A day after the third injection, half of theanimals received a sublethal dose of X-rays (2.58 Gy; the stress factor). A day after the last injection, the animals were sacrificed through the section of thecarotid artery. Ultrastructural investigations were performed at the spleen level. The analysis of the ultrastructural features from the spleen level enabledthe following observations: the single action of the TiO2 or TiO2-Ag nanoparticles induced an inflammatory process, but in the presence of X-rays, they manifested a slight protective effect; the TiO2-Ag nanoparticles, encapsulated in liposome, manifested a strong radioprotective effect, but the endocapsulation process is not optimal and has to be improved; the presence of a protective effect at the spleen level can suggest that the TiO2-Ag nanoparticles can be used to enhance the organism resistance in case of carcinogenic treatment (in animal or in homo)