Pacheco Torres, Víctor Hugo

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The Open-Access Journal for the Basic Principles of Diffusion Theory, Experiment and ApplicationAssessment of GABARAP self-association by its diffusion propertiesVı´ctor Hugo Pacheco TorresInstitute of Complex Systems (ICS-6), Forschungszentrum Ju¨lich, Ju¨lich, Germanyvpachecotorres@googlemail.comGamma-aminobutyric acid type A-receptor-associated protein (GABARAP) belongs to a family ofsmall ubiquitin-like adaptor proteins implicated in intracellular vesicle trafficking and autophagy.Biochemical evidence, as well as observations from X-ray crystallography, supports the view thatGABARAP shows a propensity to oligomerize in solution. Here, we have used diffusion-ordered nu-clear magnetic resonance spectroscopy (DOSY-NMR) to study the temperature and concentration de-pendence of the diffusion properties of GABARAP. Our data suggest the presence of distinct conforma-tional states and provide support for self-association of GABARAP molecules. Assuming a monomer–dimer equilibrium, the mass fraction of the dimer could be determined for every condition. Based on aseries of 1H-15N-heteronuclear single quantum coherence (HSQC) NMR spectra recorded at differenttemperatures, we propose residues potentially involved in GABARAP self-interaction.

Assessment of GABARAP self-association by its diffusion properties

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Assessment of GABARAP self-association by its diffusion properties

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