Mironov, O. A.

A.O. Kovalevsky Institute of Marine Biological Research Open access repository

Национальная Академия наук Украины
Институт биологии южных морей им. А.О. Ковалевского
Управление по делам семьи и молодежи СГГА
Тезисы V Международной
научно-практической конференции молодых ученых
по проблемам водных экосистем
(24  27 сентября, 2007) 
Севастополь
2007 
PONTUS EUXINUS  2007 
μg·g-1 FW in gills). These proteins had been consisted of two major isoforms: 
seasonal independent MT-1 and reduced in summer MT-2. MTs accumulate
above 40% of Cu but less then 5% of total Zn in tissues with few exceptions. 
MTs bound almost all Cd in the tissues, mostly in digestive gland, whilst a ratio
Cd:(Zn+Cu) in MTs was less then 1:20. The extremely enlarged Zn level (about 
four times) and reduced Cu level in tissues and MTs in summer comparing to 
autumn was reflected. The Zn:Cu ratio in MTs was 2:1 in autumn and 11:1 in 
summer. Zn was also accumulated in summer in other than MTs soluble 
compounds. PCA analysis revealed the inversely relation of Zn level in tissue 
and its compounds to its content in water while all Cu parameters in tissues were
correlated with its content in water. The UV-spectra as well as chromatographic 
analysis indicated the loss of specific features of MTs in summer in comparison
to autumn. The up-regulation of Zn level in summer seems to be a primary
reason for seasonal changing of MTs. 
This work has been granted by Ukrainian-French program Dnipro The
assessment of freshwater pollution based on use of metallothioneins of
mollusks.
Mironov O. A. 
OIL HYDROCARBONS ON THE PERIPHYTON OF THE 
HYDROTECHNICAL CONSTRUCTIONS 
Sevastopol State Technical University
Department of  applied ecology and labour protection.
Vast literature is devoted to co-operation of marine organisms with oil 
contamination including summarizing works. The particular materials about the
accumulation of oil hydrocarbons by marine hydrobionts are given in them, e.g. 
about getting of oil into the organisms. The question about superficial
contamination of marine flora and fauna practically is not sanctified. Such
information is known about catastrophic overflows of oil emphasizes mostly on
birds and mammals. The information about superficial contamination of other
representatives of marine biota is absent. At the same time it is possible to look
how marble crabs get out on off-shore stone and again get into the water,
repeatedly crossing oil pellicle. Tracks of oil contamination are well visible on
rocks overgrowings and hydrotechnical constructions. However quantitative 
information about such contamination is absent.  In this connection the purpose
140 
PONTUS EUXINUS  2007 
of the real work was a study of oil hydrocarbons on perifytone of hydrotechnical
constructions of Artilleriyskaya bay and adjoining area of coast, which is under
the large recreational and economic loading.
Samples were taken from submarine part of concrete embankment in May
July, 2007 on 17 points by a hand scraper from grounds 10x10 cm. Selection was
done on formed in a boundary layer «sea-atmosphere» overgrowings, which
were represented the «brush» of water-plants-macrophytes length of which as a
result of wave influence does not exceed 12 cm. Samples were dried on a
filtration paper. The hinge-plate of 10g was taken from each sample and was 
extracted by CCl4 with subsequent determination of oil hydrocarbons by the method of infra-red spectrometry on the device of SPECORD-75IR. 
The maximal value of oil hydrocarbons (0,33 mg/g) was fixed at the station #7 in
angular part of bay, where the place of mooring of city cutters and ferries is situated, 
and the flow of the thundershower sewage system goes out. On the extreme east and 
western banks of bay a concentration of oil hydrocarbons was 0,020,08 mg/g and
0,015  0,03 mg/g accordingly. Correlation dependence is traced between the amount
of oil hydrocarbons and general amount of matters extracted by CCl4. The coefficientof correlation was 0,552. At the same time there is considerable oscillation of
percentage of oil hydrocarbons in general mass of matters extracted by CCl4from 4% to 41%. 
Romanchuk L. D., Falfushуnska H. I., Semyrosum T. K., Chorna Z. P. 
ACCUMULATION OF HEAVY METALS IN FROGS FROM TWO
WETLANDS 
Ternopil V. Hnatiuk National Teachers Training University
Kryvonosa Str., 2, Ternopil; 46027, Ukraine 
stolyarok@rambler.ru, halynkaF@ukr.net
Up to now, few studies have been carried out regarding heavy metal
accumulation in amphibian species in natural populations and, to our knowledge
such information concerning Ukrainian amphibians is lacking at all. Therefore, 
the aim of this study was to elucidate the seasonal and spatial fluctuations of 
heavy metals content in the liver of the frog Rana ridibunda in the urban and 
rural sites in the Dnister basin near Ternopil city, to examine the possibility of
using it as a bioindicator species of water quality and also to examine the 
functional state of the organism. 
141 


Oil hydrocarbons on the periphyton of the hydrotechnical constructions

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Oil hydrocarbons on the periphyton of the hydrotechnical constructions

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