DEVRIES, HJC

GIJSBERS, G

MIDDELKOOP, E

VANMARLE, J

WESTERHOF, W

WILDEVUUR, CHR

ZEEGELAAR, JE

In full-thickness skin wounds dermal regeneration usually fails, resulting in scar formation and wound contraction. We studied dermal regeneration by implantation of collagenous matrices in a human punch biopsy wound model.Matrices were made of native bovine collagen I fibres, and either hyaluronic acid, fibronectin, or elastin was added. Matrices were placed in 6-mm punch biopsy holes in seven patients (biopsies were used for the grafting of leg ulcers), and covered with a protective semi-permeable polyether urethane membrane. Histology, wound contraction and dermal architecture were studied. Dermal architecture was evaluated using a recently developed laser scatter technique.All collagen matrices showed a tendency to reduce wound contraction, compared with control wounds; elastin- and fibronectin-treated matrices showed significantly less contraction than control wounds. Only the addition of elastin had a clear beneficial effect on dermal architecture; collagen bundles were more randomly organized, compared with control wounds, and wounds treated with collagen matrices coated with fibronectin or hyaluronic acid, or without coating.We conclude that the punch biopsy wound model provides important information on dermal regeneration in humans, Native collagen matrices with elastin contributed to dermal regeneration and reduced wound contraction, in contrast with matrices coated with fibronectin or hyaluronic acid, or without coating. Future clinical studies of large-area, full-thickness wounds will be required to establish their clinical relevance for leg ulcer and burn treatment.</p

REDUCED WOUND CONTRACTION AND SCAR FORMATION IN PUNCH BIOPSY WOUNDS - NATIVE COLLAGEN DERMAL SUBSTITUTES - A CLINICAL-STUDY

Abstract

Similar works

Full text

Available Versions

Proceedings - University of Groningen