In this study, metabolic engineering strategies and fermentation Bacillus subtilis. B. subtilis, as well as the culture conditions on amorphadiene production. optimizations were applied to improve the production of amorphadiene in The former includes increasing precursor supply and enhancing expression of heterologous Amorphadiene Synthase (ADS) in , and the latter explored the effects of different medium omponents as well as the culture conditions on amorphadiene production