Allen, JD

Dalton, WS

de Jong, MC

Maliepaard, M

Pijnenborg, ACLM

Ross, DD

Scheffer, GL

Schellens, JHM

Scheper, RJ

Schroeijers, AB

van der Valk, P

van Gastelen, MA

Tumor cells may display a multidrug resistant phenotype by overexpression of ATP-binding cassette transporters such as multidrug resistance (,MDR1) P-glycoprotein, multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP). The presence of BCRP has thus far been reported solely using mRNA data. In this study, me describe a BCRP-specific monoclonal antibody, BXP-34, obtained from mice, immunized with mitoxantrone-resistant, BCRP mRNA-positive MCF-7 MR human breast cancer cells. BCRP was detected in BCRP-transfected cells and in several mitoxantrone- and topotecan-selected tumor cell sublines. Pronounced staining of the cell membranes showed that the transporter is mainly present at the plasma membrane, In a panel of human tumors, including primary turners as well as drug-treated breast cancer and acute myeloid leukemia samples. BCRP was low or undetectable. Extended studies will be required to analyze the possible contribution of BCRP to clinical multidrug resistance.</p

Dissertations of the University of Groningen

Breast cancer resistance protein is localized at the plasma membrane in mitoxantrone- and topotecan-resistant cell lines

Breast cancer resistance protein is localized at the plasma membrane in mitoxantrone- and topotecan-resistant cell lines

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Dissertations of the University of Groningen