Castro, Paula M.L.

Ferreira, Maria I.M.

Fátima Carvalho, Maria de

Janssen, Dick B.

Moreira, Irina S.

English

Dissertations of the University of Groningen

   University of GroningenDegradation of fluorobenzene by a Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catecholFátima Carvalho, Maria de; Ferreira, Maria I.M.; Moreira, Irina S.; Castro, Paula M.L.;Janssen, Dick B.IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:2007Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Fátima Carvalho, M. D., Ferreira, M. I. M., Moreira, I. S., Castro, P. M. L., & Janssen, D. B., (2007).Degradation of fluorobenzene by a Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol andcatechol, 1 p.CopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Download date: 04-06-2022hnolo1DoMMatPbeGTbfdbsrcFcdcrftLwpIc1ggtclbdAbstracts / Journal of Biotec4.egradation of fluorobenzene by a Rhizobiales strain F11 viartho cleavage of 4-fluorocatechol and catecholaria de Fátima Carvalho a,∗, Maria I.M. Ferreira b, Irina S.oreira a, Paula M.L. Castro a, Dick B. Janssen bEscola Superior de Biotecnologia, Universidade Católica Por-uguesa, Rua Dr. António Bernardino de Almeida, 4200-072orto, PortugalDepartment of Biochemistry, Groningen Biomolecular Sci-nces and Biotechnology Institute, University of Groningen,roningen, Netherlandshe aerobic metabolism of FB was investigated using a pureacterial culture that was isolated from a consortium enrichedrom a contaminated site in northern Portugal. This strain,esignated F11 and phylogenetically related to the Rhizo-iales order, can grow on FB as a sole carbon and energyource under aerobic conditions. To investigate the catabolicoute for FB, activities of enzymes of the ortho- and meta-leavage pathways were measured in cell-free extracts of strain11 grown on FB. Activity for the key enzyme of the meta-leavage pathway – catechol 2,3-dioxygenase – was nevertdgy 131S (2007) S242–S249 S249etected, while enzymes of the ortho-cleavage pathway – cate-hol 1,2-dioxygenase, muconate cycloisomerase, maleylacetateeductase and 3-oxoadipate:succinyl-coenzyme A (CoA) trans-erase – were present in the cell-free extracts. This indicatedhat FB is converted via ortho-cleavage pathway. HPLC andC–MS analysis showed that both 4-fluorocatechol and catecholere transiently present in the culture medium during the earlyhase of fluorobenzene degradation by F11 cell suspensions.nhibition experiments with 3-fluorocatechol showed that thisompound is a strong inhibitor of FB degradation and of catechol,2-dioxygenase activity, whereas 4-fluorocatechol supportedrowth and was converted by cell-free extracts. The results sug-est that fluorobenzene is degraded by initial dioxygenationo yield either 4-fluorocatechol after subsequent reduction, oratechol with concomitant defluorination. The main metabo-ite is proposed to be 4-fluorocatechol, which is convertedy ortho cleavage to 3-fluoro-cis,cis-muconate and subsequentefluorination yields maleylacetate that is channeled into thericarboxylic acid cycle via 3-oxoadipate.oi:10.1016/j.jbiotec.2007.07.451

Degradation of fluorobenzene by a Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

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Degradation of fluorobenzene by a Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

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