Breum, Solvej Østergaard

Cirera, S.

Dürrwald, R.

Heegaard, Peter M. H.

Podolska, A.

Schlegel, M.

Skovgaard, Kerstin

Vasby, D.

Swine influenza is a highly infectious respiratory disease in pigs caused by influenza A virus. Activation of a frontline of pattern-recognition receptors (PRRs) expressed by epithelial cells as well as immune cells of the upper respiratory tract, leads to a potent type 1 interferon (IFN) release and simultaneous proinflammatory cytokine expression. A transient induction of cytokines is required for an efficient antiviral defence; however, an over-reactive and prolonged inflammatory response may lead to excessive infiltration of immune cells, contributing to immunopathology of the infected lung. Thus, this response must be tightly regulated. Recently, microRNA (miRNA) has been proposed to play an important role in modulating and fine tuning the innate immune response in order to avoid such harmful overreactions. Little is known about the significance of miRNA regulation in the lung during acute influenza A infection. The present work aimed of providing a better understanding of the involvement of innate immune factors including miRNA in the host response to establishment and progression of influenza virus infection. Twenty pigs were challenged by aerosol containing H1N2 (A/swine/Denmark/12687/03) influenza virus. Expression of mRNA coding for cytokines, chemokines, pattern recognition receptors and other antiviral effector molecules were quantified in lung tissue at different time points after challenge (24h PI, 72h PI, and 14days PI). Likewise, microRNA in the lung tissue was quantified at the same time points. Our results demonstrate a significant regulation of several microRNAs and their targeted mRNA in the lungs of pigs during acute influenza

Online Research Database In Technology

Expression of microRNAs and innate immune factor genes in lung tissue of pigs infectedwith influenza virus (H1N2) - DTU Orbit (06/08/2016) Expression of microRNAs and innate immune factor genes in lung tissue of pigs infected with influenza virus (H1N2)Swine influenza is a highly infectious respiratory disease in pigs caused by influenza A virus. Activation of a frontline ofpattern-recognition receptors (PRRs) expressed by epithelial cells as well as immune cells of the upper respiratory tract,leads to a potent type 1 interferon (IFN) release and simultaneous proinflammatory cytokine expression. A transientinduction of cytokines is required for an efficient antiviral defence; however, an over-reactive and prolonged inflammatoryresponse may lead to excessive infiltration of immune cells, contributing to immunopathology of the infected lung. Thus,this response must be tightly regulated. Recently, microRNA (miRNA) has been proposed to play an important role inmodulating and fine tuning the innate immune response in order to avoid such harmful overreactions.  Little is known about the significance of miRNA regulation in the lung during acute influenza A infection. The present workaimed of providing a better understanding of the involvement of innate immune factors including miRNA in the hostresponse to establishment and progression of influenza virus infection.  Twenty pigs were challenged by aerosol containing H1N2 (A/swine/Denmark/12687/03) influenza virus. Expression ofmRNA coding for cytokines, chemokines, pattern recognition receptors and other antiviral effector molecules werequantified in lung tissue at different time points after challenge (24h PI, 72h PI, and 14days PI). Likewise, microRNA in thelung tissue was quantified at the same time points. Our results demonstrate a significant regulation of several microRNAsand their targeted mRNA in the lungs of pigs during acute influenza. General informationState: PublishedOrganisations: National Veterinary Institute, Section for Immunology and Vaccinology, Section for Virology, SallingvejDyreklinik, IDT-Biologika GmbH, University of CopenhagenAuthors: Skovgaard, K. (Intern), Cirera, S. (Ekstern), Vasby, D. (Ekstern), Podolska, A. (Ekstern), Breum, S. Ø. (Intern),Schlegel, M. (Ekstern), Dürrwald, R. (Ekstern), Heegaard, P. M. H. (Intern)Publication date: 2012Event: Abstract from International Workshop on Small RNA in Cancer, Inflammation and Aging, Copenhagen, Denmark.Main Research Area: Technical/natural sciencesElectronic versions: prod11376397868186.kerstin_CIA Source: dtuSource-ID: u::8350Publication: Research - peer-review › Conference abstract for conference – Annual report year: 2013 

Expression of microRNAs and innate immune factor genes in lung tissue of pigs infected with influenza virus (H1N2)

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Expression of microRNAs and innate immune factor genes in lung tissue of pigs infected with influenza virus (H1N2)

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