The objective of this project is to evaluate MoDOT’s alternate lane shift sign configuration for work zones. The single signproposed by MoDOT provides the traveler with enough information to let them know that all lanes are available to shift around thework zone, whereas the MUTCD signs require drivers to see two signs. This research simulation project evaluates the drivers’ laneshifting performance and acceptance of the alternate lane shift sign proposed by MoDOT to be used on work zones as compared tothe MUTCD lane shift signs. Based on the study results, no difference was observed between MUTCD lane shift sign andMoDOT lane shift sign lane shift patterns with respect to driving patterns. In summary, statistical data analysis clearlydemonstrated that there was not a noticeable, statistical difference between lane change patterns of drivers in the MoDOT alternatesigns with MUTCD signs in the work zone