Gluten proteins and rheology of Mexican bread wheats as affected by environmental and genotypic factors

Abstract

The objective of this study was to determine the effect of environmental and genotypic factors on industrial quality and on the monomeric and polymeric protein contents and ratio of 24 rainfed bread wheat recombinant lines. The cultivation was done in five environmental conditions generated by agronomic management in the autumn-winter 2006/2007 cycle, at Roque, Guanajuato, Mexico. The mixing time (TMA), strength (ALVW) and extensibility (ALVPL) of the dough, gliadin (50PS) and glutenin (50PI) rich fractions, and their ratio (50PS/50PI), were evaluated. The best combinations of high and low molecular weight glutenins for TMA and ALVW were 1, 17+18, 5+10/Glu-A3c, Glu-B3g, Glu-D3b; 1, 17+18, 5+10/Glu-A3c, Glu-B3h, Glu-D3b; and 2*, 17+18, 5+10/Glu-A3c, Glu-B3g, Glu-D3b; for ALVPL it was 2*, 17+18, 2+12/Glu-A3e, Glu-B3h, Glu-D3b; and for 50PS they were 2*, 17+18, 2+12/Glu-A3e, Glu-B3h, Glu-D3b; and 1, 17+18, 5+10/Glu-A3e, Glu-B3h, Glu-D3b. The 50PS/50PI ratio was higher in genotypes with 2*, 17+18, 2+12/Glu-A3e, Glu-B3g, Glu-D3b. TMA is higher when temperature increases, and the best ALVPL is found under normal environmental conditions. Gliadin fraction and the 50PS/50PI ratio are higher when sulphur fertilization is done, and 50PI increases with drought and higher temperatures during grain-filling stage.El objetivo de este estudio fue conocer el efecto de factores ambientales y genotípicos sobre los parámetros de calidad industrial y sobre la cantidad y relación de proteínas monoméricas y poliméricas del gluten en 24 líneas recombinantes de trigos harineros de temporal. El cultivo se desarrolló en cinco condiciones ambientales generadas por manejo agronómico, ciclo otoño-invierno 2006/2007, en Roque, Guanajuato, México. Se evaluaron el tiempo de amasado (TMA), fuerza (ALVW), extensibilidad (ALVPL) de la masa, fracción rica en gliadina (50PS) y en glutenina (50PI), y su relación (50PS/50PI). Las mejores combinaciones de gluteninas de alto y bajo peso molecular para TMA y ALVW fueron los genotipos con 1, 17+18, 5+10/Glu-A3c, Glu-B3g, Glu-D3b; 1, 17+18, 5+10/Glu-A3c, Glu-B3h, Glu-D3b, y 2*, 17+18, 5+10/Glu-A3c, Glu-B3g, Glu-D3b; para ALVPL, 2*, 17+18, 2+12/Glu-A3e, Glu-B3h, Glu-D3b; para 50PS, 2*, 17+18, 2+12/Glu-A3e, Glu-B3h, Glu-D3b; y 1, 17+18, 5+10/Glu-A3e, Glu-B3h, Glu-D3b. La relación 50PS/50PI fue mayor en genotipos con 2*, 17+18, 2+12/Glu-A3e, Glu-B3g, Glu-D3b. El TMA es mayor cuando aumenta la temperatura y la mejor ALVPL se obtiene en el ambiente bajo condiciones normales. La fracción 50PS y la relación 50PS/50PI son mayores cuando se realiza la fertilización con azufre, y se obtiene incremento de 50PI con riego limitado y aumento de temperaturas durante el llenado de grano.The objective of this study was to determine the effect of environmental and genotypic factors on industrial quality and on the monomeric and polymeric protein contents and ratio of 24 rainfed bread wheat recombinant lines. The cultivation was done in five environmental conditions generated by agronomic management in the autumn-winter 2006/2007 cycle, at Roque, Guanajuato, Mexico. The mixing time (TMA), strength (ALVW) and extensibility (ALVPL) of the dough, gliadin (50PS) and glutenin (50PI) rich fractions, and their ratio (50PS/50PI), were evaluated. The best combinations of high and low molecular weight glutenins for TMA and ALVW were 1, 17+18, 5+10/Glu-A3c, Glu-B3g, Glu-D3b; 1, 17+18, 5+10/Glu-A3c, Glu-B3h, Glu-D3b; and 2*, 17+18, 5+10/Glu-A3c, Glu-B3g, Glu-D3b; for ALVPL it was 2*, 17+18, 2+12/Glu-A3e, Glu-B3h, Glu-D3b; and for 50PS they were 2*, 17+18, 2+12/Glu-A3e, Glu-B3h, Glu-D3b; and 1, 17+18, 5+10/Glu-A3e, Glu-B3h, Glu-D3b. The 50PS/50PI ratio was higher in genotypes with 2*, 17+18, 2+12/Glu-A3e, Glu-B3g, Glu-D3b. TMA is higher when temperature increases, and the best ALVPL is found under normal environmental conditions. Gliadin fraction and the 50PS/50PI ratio are higher when sulphur fertilization is done, and 50PI increases with drought and higher temperatures during grain-filling stage

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