Friedberg, Errol C.

Reagan, Michael S.

Siede, Wolfram

English

College of Saint Benedict and Saint John’s University: DigitalCommons@CSB/SJU

College of Saint Benedict and Saint John's University DigitalCommons@CSB/SJU Biology Faculty Publications Biology 3-1995 Characterization of a Saccharomyces cerevisiae strain deleted for the RAD27 gene; a structural homolog of the RAD2 nucleotide excision repair gene Michael S. Reagan College of Saint Benedict/Saint John's University, mreagan@csbsju.edu Wolfram Siede Errol C. Friedberg Follow this and additional works at: https://digitalcommons.csbsju.edu/biology_pubs  Part of the Biology Commons, and the Molecular Genetics Commons Recommended Citation Reagan MS, Pittenger C, Siede W, Friedberg EC. 1995. Characterization of a Saccharomyces cerevisiae strain deleted for the RAD27 gene; a structural homolog of the RAD2 nucleotide excision repair gene. Journal of Cellular Biochemistry 59 Suppl. 21A: 303. This is the peer reviewed version of the following article: Reagan MS, Pittenger C, Siede W, Friedberg EC. 1995. Characterization of a Saccharomyces cerevisiae strain deleted for the RAD27 gene; a structural homolog of the RAD2 nucleotide excision repair gene. Journal of Cellular Biochemistry 59 Suppl. 21A: 303, which has been published in final form at http://dx.doi.org/10.1002/jcb.240590607. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This is the peer reviewed version of the following article: Reagan MS, Pittenger C, Siede W, Friedberg EC. 1995. Characterization of a Saccharomyces cerevisiae strain deleted for the RAD27 gene; a structural homolog of the RAD2 nucleotide excision repair gene. Journal of Cellular Biochemistry 59 Suppl. 21A: 303, which has been published in final form at http://dx.doi.org/10.1002/jcb.240590607. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. CHARACTERIZATION OF A Saccharomyces cerevisiae STRAIN DELETED FOR THE RAD27 GENE; A STRUCTURAL HOMOLOG OF THE RAD2 NUCLEOTIDE EXCISION REPAIR GENE  Michael S. Reagan Wolfram Siede  Errol C. Friedberg Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75235   We have constructed a strain of the yeast Saccharomyces cerevisiae which is deleted of the YKL510 open reading frame, initially identified in chromosome XI as a homolog of the RAD2 nucleotide excision repair gene (A. Jacquier, P. Legrain, and B. Dujon, Yeast 8:121-132, 1992). The mutant strain exhibits moderate sensitivity to ultraviolet (UV) light and severe sensitivity to the alkylating agents methylmethane sulfonate, methylnitrosourea, and N-methyl-N'-nitro-N-nitrosoguanidine, but is not sensitive to ionizing radiation. We have renamed the YKL510 open reading frame the RAD27 gene, in keeping with the accepted nomenclature for radiation-sensitive yeast mutants. Epistasis analysis indicates that the gene is in the RAD6 group of genes involved in DNA damage tolerance and mutagenesis. The mutant strain is temperature-sensitive, with the cells arresting uniformly as large-budded dumbell shaped cells containing a single nucleus with a 2N DNA content. The strain also exhibits increased plasmid loss and increased spontaneous mutagenesis, but is normal with respect to UV-induced mutagenesis. Transcript levels of the RAD27 gene are cell cycle regulated in a manner similar to that of several other genes whose products are known to be involved in DNA replication. These data are consistent with the Rad27 protein having a role in both DNA replication and DNA repair. 

Characterization of a \u3ci\u3eSaccharomyces cerevisiae\u3c/i\u3e strain deleted for the RAD27 gene; a structural homolog of the RAD2 nucleotide excision repair gene

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Characterization of a \u3ci\u3eSaccharomyces cerevisiae\u3c/i\u3e strain deleted for the RAD27 gene; a structural homolog of the RAD2 nucleotide excision repair gene

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