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AC Billi

Alexandra Sapetschnig

B Yu

C Ender

C Frokjaer-Jensen

C Merritt

CC Conine

CD Malone

D Updike

DM Pavelec

DP Bartel

E Cuppen

E Yigit

Eric A. Miska

Eugene Berezikov

F Simmer

G Hutvagner

G Wang

GN Cox

H Tabara

HM Kurth

J Li

J Pak

JC van Wolfswinkel

JG Ruby

JI Gent

JJ Vasale

JM Claycomb

Josien C. van Wolfswinkel

K Aoki

K Miyoshi

K Saito

KL Tkaczuk

Leonie M. Kamminga

LM Kamminga

Lucas J. T. Kaaij

M Ghildiyal

M Kusch

Maartje J. Luteijn

Marloes P. Bagijn

MD Horwich

MP Bagijn

NL Vastenhouw

P Flicek

PJ Batista

PP Das

RC Lee

René F. Ketting

RF Ketting

RS Kamath

S Guang

S Kawaoka

S Kennedy

SK Kim

SL Ameres

Stuart K. Kim

T Han

T Sijen

TA Montgomery

TF Duchaine

VV Vagin

Y Huang

Y Kirino

Y Tian

Z Zhang

PLoS Genetics

PubMed

Crossref

Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans

<div>RNA interference (RNAi)–related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 3′ ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA–methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 3′-uridylation frequencies. Within the 26G RNA class, we find that specifically ERGO-1–bound 26G RNAs are modified by HENN-1, while ALG-3/ALG-4–bound 26G RNAs are not. Global gene expression analysis of henn-1 mutants reveals mild effects, including down-regulation of many germline-expressed genes. Our data suggest that, apart from direct effects of reduced 26G RNA levels of henn-1 on gene expression, most effects on global gene expression are indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways. </div

Leonie M. Kamminga (150094)

Josien C. van Wolfswinkel (150099)

Maartje J. Luteijn (150106)

Lucas J. T. Kaaij (150113)

Marloes P. Bagijn (150120)

Alexandra Sapetschnig (150128)

Eric A. Miska (41828)

Eugene Berezikov (9900)

René F. Ketting (6711)

The Francis Crick Institute

Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans

Kamminga, L.M.

Wolfswinkel, J.C. van

Luteijn, M.J.

Kaaij, L.J.T.

Bagijn, M.P.

Sapetschnig, A.

Miska, E.A.

Berezikov, E.

Ketting, R.F.

Radboud Repository

RNA interference (RNAi)-related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA) co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 3' ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA-methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 3'-uridylation frequencies. Within the 26G RNA class, we find that specifically ERGO-1-bound 26G RNAs are modified by HENN-1, while ALG-3/ALG-4-bound 26G RNAs are not. Global gene expression analysis of henn-1 mutants reveals mild effects, including down-regulation of many germline-expressed genes. Our data suggest that, apart from direct effects of reduced 26G RNA levels of henn-1 on gene expression, most effects on global gene expression are indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways

Leonie M Kamminga

Josien C van Wolfswinkel

Maartje J Luteijn

Lucas J T Kaaij

Marloes P Bagijn

Eric A Miska

René F Ketting

Directory of Open Access Journals

Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans.

NARCIS 

van Wolfswinkel, J.C.

Kaaij, L.J.

Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans

Kamminga Leonie M.

van Wolfswinkel Josien C.

Luteijn Maartje J.

Kaaij Lucas J. T.

Bagijn Marloes P.

Sapetschnig Alexandra

Miska Eric A.

Berezikov Eugene

Ketting René F.

Public Library of Science (PLOS)

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The rde1 gene, RNA interference, and transposon silencing in C.

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Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans

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Directory of Open Access Journals

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