用不同方法对巴马火麻油进行甲酯化处理,用气相色谱-质谱联用(GC-MS)对其脂肪酸成份、含量进行分析。采用盐酸-甲醇溶液法、硫酸-甲醇溶液法、氢氧化钾-甲醇溶液法以及硫酸-氢氧化钾-甲醇溶液法处理火麻油,用GC-MS对其中的脂肪酸组成、含量进行分析、比较。结果表明,4种甲酯化方法分别鉴定出6,7,9和9种脂肪酸,分别占火麻油总量的96.62%,98.15%,98.15%和95.82%。4种方法测定出的棕榈酸含量分别为17.18%,10.83%,18.79%和21.63%;亚油酸含量分别为33.96%,46.73%,0.32%和31.76%;亚麻酸含量分别为38.46%,36.76%,35.14%和33.11%;氢氧化钾-甲醇溶液法测出含33.13%的8,11-十八碳二烯酸。巴马火麻油富含不饱和脂肪酸,特别是亚麻酸含量较高,是高品质的营养油。The fatty acids in bama hemp seed oil were estirified by estirificition methods and analyzed by GC-MS. The hemp oil was estirified by hydrochloric acid-methanol solution method, sulfuric acid methanol solution method, potassium hydroxide-methanol solution method, sulfuric acid-potassium hydroxide-methanol solution method and analyzed by GCMS. The results showed that 6, 7, 9 and 9 fatty acids were identified by the four methods. The contents of the identified fatty acids accounted for 96.62%, 98.15%, 98.15% and 95.82%, respectively. Accord to the different analysis methods, each of the contained volume of hexadecanoic acid was 17.18%, 10.83%, 18.79% and 21.63%; 9, 12-octadecadienoic acid was 33.96%, 46.73%, 0.32% and 31.76%; Linolenic acid was 38.46%, 36.76%, 35.14% and 33.11%. 8, 11-octadecadienoic acid was identified by sulfuric acid-potassium hydroxide-methanol solution method, the contents of the eicosanoic acid accounted for 33.13%. The results showed that the hemp seed oil was rich in unsaturated fatty acids, the bama hemp seed oil was health oil and high nutritional value.国家自然科学基金项目(21165003)； 广西民族大学实验技术创新与实验管理研究项目,广西民族大学科研基金资助课题(2016MDYB030)； 广西民族预科教育基地重点项目(2016A006
