目的:以不同产地的毛果鱼藤藤茎为研究对象,建立中药毛果鱼藤藤茎HPLC指纹图谱;检测不同极性部位抑制癌细胞增殖活性,为科学评价和有效控制毛果鱼藤的质量及其抗肿瘤活性提供科学依据。方法:微波法提取毛果鱼藤藤茎中的有效成分,采用高效液相色谱法建立毛果鱼藤藤茎的指纹图谱。色谱柱:Wonda SilTM-C18(5μm,4.6 mm×250 mm);流动相:乙腈(A)-0.5%醋酸溶液(B),梯度洗脱(0~20 min,40%A→60%A;20~30 min,60%A;30~45 min,60%A→80%A;45~60 min,80%A);流速:1.0 m L·min-1;检测波长:296 nm;柱温:35℃;进样量:10μL;采用MTT法,考察毛果鱼藤不同极性部位对NCIH460人肺癌细胞、SW480人结肠癌细胞、SGC7901人胃癌细胞、Hela人宫颈癌细胞的生长抑制作用。结果:建立了中药毛果鱼藤藤茎HPLC特征指纹图谱共有模式,标定了16个共有峰,10个批次毛果鱼藤藤茎药材指纹图谱经国家药典规定的相似度计算软件计算,整体相似度良好;抗癌试验结果表明,毛果鱼藤藤茎的石油醚、氯仿、乙酸乙酯、正丁醇提取部位均能显示不同程度的抑制癌细胞生长的作用,并呈量效关系,且石油醚、氯仿部位表现出较强的抗癌活性,氯仿部位对Hela人宫颈癌细胞的IC50值为0.09 mg·m L-1。结论:毛果鱼藤藤茎高效液相指纹图谱的相似度较高,其不同溶剂提取物抑制癌细胞生长作用的研究表明,石油醚和氯仿提取部分活性较强。Abstract Objective: To study the HPLC fingerprint and antitumor activity of different polar parts extracted from
crude stem of Derris eriocarpa How from Guangxi，and establish the scientific system to evaluate the quality of the
medicinal materials． Methods: The fingerprints were built by HPLC， the WondaSilTM － C18
( 5 μm，4. 6 mm × 250
mm) column were used with the mobile phase under a linear gradient( 0 － 20 min， 40% A→60% A; 20 － 30 min，
60%A; 30 － 45 min， 60% A→80% A; 45 － 60 min，80% A) which consisted of acetonitrile( A) and 0. 5% acetic
acid solution( B) at the flow rate of 1. 0 mL·min － 1 ． The 296 nm UV detection wavelength and the 35 ℃ column
temperature was used． The viability inhibition effect of different extracts from stem of Derris eriocarpa How on tumor
cells( lung cancer cell NCI － H460，colon cancer cell SW480，gastric cancer cell SGC7901 and cervical cancer cell
Hela) was measured by the MTT assay． Ｒesults: The common fingerprints were founded with sixteen HPLC peaks from ten batches of samples of Derris eriocarpa How by the overall similarity software specified in the national pharmacopeia．
The overall similarity was good． The antitumor test showed that ligarine，chloroform，acetidin，and n － butyl
alcohol from different polar parts extracted from the crude stem inhibited the growth of cancer cells in a dose －
dependent manner． It was notable that the parts of ligarine and chloroform extracts exhibited strong antitumor activity
and the IC50value of chloroform extracts on Hela cell was 0. 09 mg·mL － 1 ． Conclusion: The specific fingerprint was
established with high similarity of Derris eriocarpa samples． The experiment of the inhibitory effect of the different
solvent extracts on cancer cell growth showed that the stronger activity part was petroleum ether and chloroform extracts．广西教育厅科研项目(YB2014101);国家民委基金(12GXZ009);广西民族大学重点项目(2012MDZD041
