カブ(Brassica rapa ssp. rapa)品種77Bで見いだされた雄性不稔性について,花の形態および遺伝様式を調査した。雄性不稔個体は可稔個体と比較して雄蕊が短く,花弁が小さかった。雄性不稔個体を77Bの可稔個体および異なる2品種と交配し,その後代の分離比を調査した結果,本雄性不稔性は劣性の一遺伝子座によって支配されていることが示された。雄性不稔性への細胞質による影響はみられなかった。また,雄性不稔および可稔個体の蕾をパラフィン切片法で薄切し,光学顕微鏡で観察した結果,雄性不稔個体における四分子期あるいはより早い時期における四分子形成の崩壊が示唆された。さらに,蕾(4-5mm)のアミノ酸解析から雄性不稔個体と比べ可稔個体でプロリン含量の著しい増加(2.7-fold)が示された。The mode of inheritance and morphology of a male sterile (ms) turnip (Brassica rapa) was studied. Morphology of the male sterile plants did not differ from that of a male fertile (mf) plant, except for the shorter stamens and smaller petals. The results of a cross between a ms plant and two cultivars of B. rapa indicated that male sterility is controlled by a single recessive gene without any cytoplasmic effect. Light microscopic observations showed no pollen grains on the inside of ms anthers, suggesting collapse of microsporogenesis at the tetrad stage or at an earlier stage in the ms plants. Analysis of the amino acid content from the 4-5mm flower buds of mf and ms plants showed a remarkable increase (2.7-fold) in proline content in the former

Fujigaki Junzo

Fujihara Eimi

Igarashi Daizo

Kenji Wakui

Komatsu Kenji

Shinohara Takashi

五十嵐 大造

和久井 健司

小松 憲治

篠原 卓

藤原 英美

藤垣 順三

カブ(Brassica rapa ssp. rapa)品種77Bで見いだされた雄性不稔性について,花の形態および遺伝様式を調査した。雄性不稔個体は可稔個体と比較して雄蕊が短く,花弁が小さかった。雄性不稔個体を77Bの可稔個体および異なる2品種と交配し,その後代の分離比を調査した結果,本雄性不稔性は劣性の一遺伝子座によって支配されていることが示された。雄性不稔性への細胞質による影響はみられなかった。また,雄性不稔および可稔個体の蕾をパラフィン切片法で薄切し,光学顕微鏡で観察した結果,雄性不稔個体における四分子期あるいはより早い時期における四分子形成の崩壊が示唆された。さらに,蕾(4-5mm)のアミノ酸解析から雄性不稔個体と比べ可稔個体でプロリン含量の著しい増加(2.7-fold)が示された。The mode of inheritance and morphology of a male sterile (ms) turnip (Brassica rapa) was studied. Morphology of the male sterile plants did not differ from that of a male fertile (mf) plant, except for the shorter stamens and smaller petals. The results of a cross between a ms plant and two cultivars of B. rapa indicated that male sterility is controlled by a single recessive gene without any cytoplasmic effect. Light microscopic observations showed no pollen grains on the inside of ms anthers, suggesting collapse of microsporogenesis at the tetrad stage or at an earlier stage in the ms plants. Analysis of the amino acid content from the 4-5mm flower buds of mf and ms plants showed a remarkable increase (2.7-fold) in proline content in the former.E7KJ00008376300論文Articledepartmental bulletin pape

和久井, 健司

2886

ワクイ, ケンジ

2887

シノハラ, タカシ

2888

フジハラ, エイミ

2889

コマツ, ケンジ

2890

イガラシ, ダイゾウ

2891

フジガキ, ジュンゾウ

Wakui, Kenji

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2893

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Tokyo University of Agriculture Repository / 東京農業大学リポジトリ

J. Agric. Sci., Tokyo Univ. Agric., 57 (4), 287-292 (2013)東京農大農学集報，57（4），287-292（2013）Genic Male Sterility in Brassica rapa ssp.rapa cv. 77BByKenji Wakui*, Takashi Shinohara*, Eimi Fujihara*, Kenji Komatsu*, Daizo Igarashi* and Junzo Fujigaki**(Received August 22, 2012/Accepted December 7, 2012)Summary：The mode of inheritance and morphology of a male sterile (ms) turnip (Brassica rapa) was studied.  Morphology of the male sterile plants did not differ from that of a male fertile (mf) plant, except for the shorter stamens and smaller petals.  The results of a cross between a ms plant and two cultivars of B. rapa indicated that male sterility is controlled by a single recessive gene without any cytoplasmic effect.  Light microscopic observations showed no pollen grains on the inside of ms anthers, suggesting collapse of microsporogenesis at the tetrad stage or at an earlier stage in the ms plants.  Analysis of the amino acid content from the 4-5 mm flower buds of mf and ms plants showed a remarkable increase (2.7-fold) in proline content in the former.Key words：genic male sterility, turnip, Brassica rapa ssp. rapa cv. 77BIntroduction　The utilization of heterosis in Brassica crops has great potential for increasing yield1-3）.  Several genetic systems have been developed for the commercial production of hybrid seed, such as cytoplasmic male sterility (CMS), genic male sterility (GMS), and self-incompatibility (SI)4）. The GMS system is not widely used because removal of 50% of segregating fertile plants in the sterile female line is required to produce seeds when a trait is controlled by a single recessive gene, which limits its utilization for the production of hybrid seed.  However, it has great poten- tial for hybrid seed production due to its particular char- acteristics : complete and stable male sterility, no nega- tive cytoplasmic effects on yield, and ease of transfer of the male sterile gene(s) to diverse genetic backgrounds5）.　Brassica rapa is an allogamous species as a result of its self-incompatible system, and contains some of the most important arable vegetable subspecies such as Chinese cabbage (ssp. pekinensis), turnip (ssp. rapa), Mizuna (ssp. japonica), Taina (ssp. chinensis) and Yukina (ssp. narinosa). Some GMS in B. rapa has been found.  One such case was described by Van der Merr (1987) for ssp. pekinensis as a dominant GMS.  A recessive GMS line of ssp. chinensis was later selected and used in F1 hybrid seed production of Brassica crops7, 8）.  Takahata et al. (1996) reported a second recessive GMS of ssp. pekinensis, the ms type, whose gene acts after microspore formation.  However, GMS turnip has not yet been reported.　We discovered a GMS plant in a population of turnip cv. 77B.  In this study, in order to characterize GMS of this cultivar, we examined the mode of inheritance, pol- len morphology and tapetum development, as well as amino acid content of flower buds in a newly found male sterile line.Materials and Methods　Five plants of B. rapa ssp. rapa cv. 77B were grown in an uncontrolled-environment greenhouse.  A male sterile (ms) plant was found from this population.  Ms plant was crossed with a male fertile (mf) plant of cv. 77B, and then their F1 plants were selfed and backcrossed to ms plants to obtain F2 and BC1 progenies, respectively.  From the segregating population, six flowers were arbitrarily selected from one individual in ms and mf, respectively, and then the sizes of stamen, petal, pistil and sepal were measured.  Measurement of size was repeated three times using different individuals in ms and mf, respectively. Difference of the size of each organ between ms and mf was tested by paired t-tests.  Also, ms plant was crossed ***Department of Bioproduction Technology, Junior College of Tokyo University of AgricultureProfessor Emeritus, Tokyo University of Agriculture288 Wakui, Shinohara, Fujihara, Komatsu, Igarashi and Fujigakiwith B. rapa ssp. rapa cv. Tsuda-kabu, and B. rapa ssp. pekinensis cv. Matsushima-shin-2gou-hakusai, respec- tively.  Their F1 plants were selfed and backcrossed to male sterile ms plants to obtain F2 and BC1 progenies, respectively.  Tsuda-kabu, as the female plants, was crossed with F1 plants produced by crossing a ms and a mf cv. 77B. Moreover, the F1 progenies of 13 plants were selfed to obtain F2 seed from the resulting plants.　At least ten flower buds of the ms and mf plants were fixed in FAA (50% ethanol, 5% glacial acetic acid, 3.7% formaldehyde) for 18-24h.  Samples were rinsed several times in 50% ethanol, dehydrated through a graded ethanol series, and stained in 100% ethanol containing 1% safranin for easy orientation of the material.  The buds were embedded in paraffin, then cut into 2-3 μm sections and stained with hematoxylene.　Frozen flower buds were triturated by pestles, and extracted three times with 80% ethanol.  The extract was desiccated by a rotary evaporator, and then re-dissolved in 1000 μl of distilled water, serving as the samples for the HPLC analysis.  The quantity of the resulting 38 amino acids in the samples was determined by an Amino Acid Analysis System (Shimadzu).Results and Discussion　The ms plants were not morphologically different from the mf plants except for the flowers.  The flower of the ms plants was characterized by small and shriveled anthers that contained no fertile pollen.  Figure 1 shows the ms and mf flowers and their constitutive organs, which differed in size (Table 1).  Ms flowers had signifi- cantly (p＜0.01) shorter stamens and sepals, and smaller petals than mf flowers.  The pistil was longer in the ms flower, which was similar to that reported by Takahata et al. (1996) for ms turnip.　All the F1 plants obtained from the cross between ms and two cultivars (cv. Tsuda-kabu and Matsushima-shin-2gou-hakusai) were mf (Table 2).  In addition to mf plants, ms plants were segregated in the F1 of the cross, ms x cv. 77B.  This indicates that the population of cv. 77B used as the male parent included heterozygous mf plants as well as homozygous mf plants.  The segregation pat- tern in the F2 and BC1 generations is shown in Table 2. Segregation data on the F2 population of ms x cv. Tsuda-kabu and ms x cv. Matsushima-shin-2gou-hakusai showed a 3 : 1 ratio (mf : ms).  In BC1 plants obtained from the cross, ms x (ms x cv. Tsuda-kabu) and ms x (ms x Matsushima-shin-2gou-hakusai), a 1 : 1 ratio of mf : ms was obtained.  This suggests that male sterility is con- trolled by a ingle recessive gene.  In order to examine the effect of cytoplasm, a cross using cv. Tsuda-kabu as the female parent and the mf progeny of ms x cv. 77B as the male parent, was carried out.  All the F1 plants showed mf.  In F2 families derived from 13 F1 plants selected randomly, five lines were composed of only mf plants, and eight lines were segregated ms plants (Table 3).  Segregation data of the eight lines was in agreement with an expected 3 : 1 (mf : ms) ratio.  The presence of five homozygous and eight heterozygous mf plants in F1 showed good agreement with an expected 1 : 1 ratio following the chi-square test ( χ2＝0.35, 0.8＜P＜0.9), con- firming that the ms turnip found in this study is con- trolled by a monogenic recessive gene.Table 1　Comparison of flower characteristics between male sterile and male fertile B. rapa lines.Fig. 1　Morphological characterization of GMS in B. rapa ssp. rapa cv. 77B. (A) and (B) show the flower and constitutive organs of mf (left) and ms (right), respectively.289Genic Male Sterility in Brassica rapa ssp. rapa cv. 77B　Light microscopic observations revealed that pollen and tapetum development of the ms plant was different from that of the mf plant at an early microspore stage (Fig. 2).  In the mf anther, microspores released from tetrads contained densely cytoplasmic cells with a nu- cleus and cell wall (Fig. 2A).  The tapetum degenerated gradually, and then completely disappeared at the late microspore stage (Fig 2B and 2C).  In ms anthers, on the other hand, microsporogenesis─even tetrad formation─could not be observed on the inside of flower buds 2-3 mm in length (Fig. 2D).  The substance inside pollen sacs was crushed and adhered (Fig. 2E), and no pollen grains were observed (Fig. 2F).  In most histologically described male sterility, microsporogenesis breaks down at the tetrad stage and sometimes at the uninucleate vacuolate microspore stage10）.  In B. rapa, breakdown of micro- Table 2　Segregation of male sterility obtained in three F1’s of ms B. rapa x cultivars and their progenies.Table 3　Segregation of male sterile plants obtained in the F2 generation of cv. Tsuda-kabu x (ms x cv. 77B).290 Wakui, Shinohara, Fujihara, Komatsu, Igarashi and Fujigakisporogenesis at the tetrad stage in male sterility was reported in var. brown sarson and yellow sarson11-13）. Cytological observation in ms cv. 77B in this study also suggests the collapse of microsporogenesis at the tetrad stage, or at an earlier stage. Indeed, male sterile stamens only had vestigia of anthers (Fig. 1B).　Analysis of amino acid content from flower buds of mf and ms plants showed a remarkable increase in proline content in the former (Fig. 3).  Proline content in flower buds (4-5 mm) of mf and ms plants accounted for 46.7% and 17.5%, respectively of the amino acid pool.  This shows that proline content in flower buds is 2.7-fold higher in mf plants than in ms plants.  Our data agrees with the result of Wu and Murry (1985), who showed that the levels of proline in the anther of a fertile line was 2-3 fold higher than that of a CMS line of petunia. Proline may function as the solute protectant in devel- oping pollen preserving the grain against unfavorable environmental conditions14, 15）.　We found a ms plant in a population of turnip cv. 77B, Fig. 3　Fifteen amino acid content in bud (2-3 mm and 4-5 mm) of ms and mf plant of B. rapa ssp. rapa cv. 77B, respectively.  ASP : aspartic acid/aspartate, THR : threonine, SER : serine, PRO : proline, GLY : glycine, ALA : alanine, VAL : valine, LEU : leucine, HIS : histidine, LYS : lysine ARG : arginine, TRP : tryptophan, MET : methionine, CYS : cysteineFig. 2　Cytological characterization of GMS in B. rapa ssp. rapa cv. 77B. (A)-(C) and (D)-(F) show microsporogenesis of mf and ms plant, respectively.  Bud size is as follows ; (A) and (D) : 2-3 mm, (B) and (E) : 4-5 mm, (C) and (F) : 6-7 mm.  ta : tapetum.  Arrow indicates the concrescence of the substance in the pollen sac.  ─ : Bar : =10 μm291Genic Male Sterility in Brassica rapa ssp. rapa cv. 77Bwhich is a European turnip cultivar resistant to clubroot16）. To date, in order to breed a new clubroot-resistant tur- nip in Japan, cv. 77B has been used as the male parent for crossing17）.  This means that this ms gene was intro- duced into a part of Japanese turnip.  On the other hand, turnip differentiated into several hundreds of local varieties in each district after it was introduced in Japan18, 19）.  In the future, another type of male sterility may be found from the gene pool of Japanese turnip varieties.  We found some Japanese cultivars showing ms traits, and their characterization is in progress.References1）　Grant I, Beverdorf W D (1985) Heterosis and combining ability estimates in spring oilseed rape (Brassica napus L.). Can. J. Genet. Cytol. 27 : 472-478.2）　Kaul M L H (1988) Male sterility in higher plants. Springer-Verlag, Berlin, 1005.3）　Brandle J L, Mcvetty P B E (1990) Geographical diversity, parental selection and heterosis in oilseed rape. Can. J. Plant Sci. 70 : 935-940.4）　Dixon D R (2006) Vegetable Brassicas and related crucifers. Crop Production Science in Horticulture Series, published by CABI, 14 : 35-72.5）　Ke L P, Sun Y Q, Hong D F, Liu P W, Yang G S (2005) Iden- tification of AFLP markers linked to one recessive genic male sterility gene in oilseed rape, Brassica napus. Plant Breed. 124 : 367-370.6）　Van dfr Merr G P (1987) Chromosomal monogenic dominant male sterility in Chinese cabbage (Brassica campestris ssp. pekinensis). Euphytica 36 : 927-931.7）　Cao S C, Li S J (1981) Breeding of the genic male sterile line of Brassica campestris L. Chinensis, Aijiaohuang. Acta Hort. Sinica 8 : 35-41.8）　Ying M, Dreyer F, Cai D, Jung C (2003) Molecular marker for genic male sterility in Chinese cabbage. Euphytica 132 : 227-234.9）　Takahata Y, Nagasaka M, Kondo H, Kaizuma N (1996) Genic male sterility in Brasscia campestris. Acta Hort. 407 : 1996.10）　Delourme R, Budar F (1999) Male sterility. Biology of Brassica coenospecies 6 : 185-216.11）　Das K, Pandey B D (1961) Male sterility in brown sarson. Indian J. Genet. Plant Breed. 21 : 185-190.12）　Chowdhury J B, Das K (1966) Male sterility in Brassica campestris var. yellow sarson. Indian J. Genet. Plant Breed. 26 : 374-380.13）　Chowdhury J B, Das K (1968) Cytomorphological studies on male sterility in Brassica campestris L. Cytologia 38 : 195-199.14）　Wu F S, Murry L E (1985) Changes in protein and amino acid content during anther development in fertile and cytoplasmic male sterility petunia. Theor. Appl. Genet. 71 : 68-73.15）　Zhang H Q, Croes A F (1983) Proline metabolism in pollen : degradation of proline during germination and early tube growth. Planta 159 : 46-49.16）　Yoshikawa H (1981) Breeding for clubroot resistance in Chinese cabbage. Chinese cabbage. Proc. first internat. symp. 405-413.17）　Yoshikawa H, Ashizawa M, Hida K, Yamagishi H (1983) Breeding of Chinese cabbage parental lines resistant to clubroot. Cruciferae Newslet. 8 : 36-27.18）　Yoshikawa H, Yui S (1991) Classification and identification of crucifer vegetables in Japan. JICA Ref. Series 4 : 97-114.19）　Shinohara S (1984) Turnip. In : Vegetable seed production technology of Japan, particulars 1 : 239-268.292 Wakui, Shinohara, Fujihara, Komatsu, Igarashi and FujigakiBrassica rapa ssp. rapa cv. 77Bにおける核遺伝子型雄性不稔性和久井健司*・篠原　卓*・藤原英美*・小松憲治*・五十嵐大造*・藤垣順三**（平成 24 年 8 月 22 日受付/平成 24 年 12 月 7 日受理）要約：カブ（Brassica rapa ssp. rapa）品種 77B で見いだされた雄性不稔性について，花の形態および遺伝様式を調査した。雄性不稔個体は可稔個体と比較して雄蕊が短く，花弁が小さかった。雄性不稔個体を 77Bの可稔個体および異なる 2 品種と交配し，その後代の分離比を調査した結果，本雄性不稔性は劣性の一遺伝子座によって支配されていることが示された。雄性不稔性への細胞質による影響はみられなかった。また，雄性不稔および可稔個体の蕾をパラフィン切片法で薄切し，光学顕微鏡で観察した結果，雄性不稔個体における四分子期あるいはより早い時期における四分子形成の崩壊が示唆された。さらに，蕾（4-5 mm）のアミノ酸解析から雄性不稔個体と比べ可稔個体でプロリン含量の著しい増加（2.7-fold）が示された。キーワード：核遺伝子型雄性不稔性，カブ，Brassica rapa ssp. rapa cv. 77B***東京農業大学短期大学部生物生産技術学科東京農業大学名誉教授

Genic Male Sterility in Brassica rapa ssp. rapa cv. 77B

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Brassica rapa ssp. rapa cv. 77B ニ オケル カクイデンシガタ ユウセイ フネンセイ

Brassica rapa ssp. rapa cv. 77B ニオケルカクイデンシガタユウセイフネンセイ

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Tokyo University of Agriculture Repository / 東京農業大学リポジトリ

Brassica rapa ssp. rapa cv. 77B ニ オケル カクイデンシガタ ユウセイ フネンセイ

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Tokyo University of Agriculture Repository / 東京農業大学リポジトリ

Brassica rapa ssp. rapa cv. 77B ニオケルカクイデンシガタユウセイフネンセイ