PREVALENCE AND IDENTIFICATION OF PATHOGENIC Leptospira IN COMMENSAL RODENT FROM MAUMERE FLORES ORIGIN

Abstract

Commensal rat have been report as main source of leptospirosis to humans, but in Indonesia the status of leptospirosis in rats commensal still not widely known. The aim of this study was to calculate the prevalence and identified pathogenic Leptospira in commensal rats in Maumere multicipality, Flores. The study was conducted from August to November 2014. Rats trapped in the perimeter and buffer area El Sai and Wuring Port, Maumere Multicipality, Flores. Detection of Leptospira in commensal rats were using PCR with specific primers 16S rRNA gene. Determination of Leptospira species by compared with the sequence of the research results with origin GenBank sequences using the BLAST program. Phylogeny tree arranged with Mega 6.2 software. The results showed that 125 commensal rat was trapped, consisted of two species, namely Rattus norvegicus and Rattus tanezumi. Five R. norvegicus positive PCR test. BLAST analysis of the results of all sequences of Leptospira synonymous with L. interrogans. Phylogenetic analysis, sequences clustered with L. interoggans Maumere. Based on the results of the research, the prevalence of Leptospira in commensal rats in Maumere multicipality were 4 percent and Leptospira that found in Maumere multiciplity was L. interogans. There was potential transmission of leptospirosis in Maumere multicipality