Link between single nucleotide polymorphism of rs266729 and rs2241766 in the ADIPOQ gene and gestational diabetes in an Iranian population

Abstract

Background: The manuscript investigates the association between adiponectin gene (ADIPOQ) polymorphisms and gestational diabetes mellitus (GDM) in an Iranian population. Methods: We designed a case-control study involving 265 normal glucose tolerant (NGT) women and 297 GDM patients. Three adiponectin single-nucleotide polymorphisms (rs1501299, rs266729 and rs2241766) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the protein level was measured by the enzyme-linked immunosorbent assay. Other biochemical parameters were assessed by conventional laboratory methods. Results: We found that rs266729 and rs2241766 were significantly associated with GDM. The genotypes analysis indicated that for rs266729, 51(61.4) of the 83 GDM patients had GG genotype, and 32(38.6) GC/CC genotype and for rs2241766, 75(58.6) of the 128 GDM patients had TT genotype, and 53(41.4) GG/GT genotype. The relation between other single-nucleotide polymorphisms (rs1501299) and GDM was not observed. Conclusion: The ADIPOQ genetic polymorphisms were associated with GDM in our studied population. © 201