Allievi, Cristina

Canciani, Elena

Colombo, Anna

Dellavia, Claudia

Ferrario, Virgilio F.

Giammattei, Michele

Firenze University Press: E-Journals

IJAE Vol. 115, n. 1/2 (Supplement), 2010
27
Histomorphometric analysis of intraoral bone grafts 
harvested by different methods
Elena Canciani, Cristina Allievi, Claudia Dellavia, Anna Colombo, Michele Giammattei, Virgilio F. 
Ferrario
 Department of Human Morphology and Biomedical Sciences “Città Studi”, University of Milan, Italy
Autogenous bone is considered the “gold standard” among graft materials for alveo-
lar ridge augmentation, because of its osteogenic, osteconductive and osteoinductive 
features. In clinical practice, autogenous bone chips can be obtained by using different 
methods, and several studies demonstrated the influence of devices used for tissue har-
vesting on bone chip morphology and vitality. 
Aim of the present study was to compare histologically and histomophometrically 
the morphological features and the vitality of bone chips harvested intraorally with five 
different methods.
Material and Methods Five young adult men were recruited for this study. Five bone 
samples were harvested from the alveolar process of each patient by means of different 
methods: 1) bone scraper, 2) stainless steel round bur, 3) stainless steel implant spiral 
bur, 4) piezoelectric device, 5) trephine bur+manual mill. Samples were processed for 
undecalcified histology and stained with toluidine blue/ pyronine G (Sigma-Aldrich, St 
Louis, MO). The sections were photographed in a Nikon light microscope (Eclipse E600) 
equipped with a calibrated digital camera (DXM1200, Nikon, Tokyo, Japan). Histomor-
phometric analyses were performed to evaluate bone fragment dimensions: fragments 
with dimensions comprised between 100µm and 1000µm (medium size fragments = MF), 
fragments smaller than 100µm (small size fragments = SF) and those greater than 1000µm 
(large size fragments = LF) were differentiated. Total area and percentage area were also 
evaluated. A stereologic method was used to calculate the percentage of vital bone.
Results Five samples for each harvesting procedure were analyzed. Bone fragments 
harvested using Trephine bur mostly resulted vital and of large dimensions. Spiral and 
implant bur produced only small and medium fragments but the percentage of non-
vital bone was higher than in the other groups. Bone scraper and piezoelectric device 
produced vital bone fragments with medium-large dimensions.
Conclusions Within the limited number of samples evaluated, results of the present 
study claim that bone scraper and piezoelectric device are the most efficacious proce-
dure on harvesting vital bone fragments.
Key words
Alveolar bone, autograft, bone vitality, histology


Histomorphometric analysis of intraoral bone grafts harvested by different methods

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Histomorphometric analysis of intraoral bone grafts harvested by different methods

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