Cardile, Venera

Carnazza, Maria Luisa

Castorina, Alessandro

D'Agata, Velia

Giunta, Salvatore

Mazzone, Venera

Firenze University Press: E-Journals

IJAE Vol. 115, n. 1/2 (Supplement), 2010
31
Effects of PACAP and VIP on hyperglycemia-induced cell 
proliferation in murine microvascular endothelial cells
Alessandro Castorina1, Salvatore Giunta1, Venera Mazzone1, Venera Cardile2, Maria Luisa Carnazza1 
and Velia D’Agata1
1 Department of Anatomy, Diagnostic Pathology, Legal Medicine, Hygiene and Public Health, University of Catania, 
Italy
2 Department of Physiological Sciences, University of Catania, Italy
Hyperglycemia is implicated both in micro- and macrovascular complications in dia-
betes mellitus. Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoac-
tive intestinal polypeptide (VIP) are two known nonclassic regulators of angiogenesis, 
although their biological role on endothelial cell proliferation remains poorly defined. 
In the present study we investigated both peptides effect on cell proliferation of murine 
microvascular endothelial cells (H5V) cultured both under normoglycemic and hyperg-
lycemic (25mM glucose) conditions for 24h, 48h, 7 and 15 days. 
Results demonstrated that high glucose treatment induced a time-dependent in-
crease in cell viability within 48h (p<0.05), which was much more evident after 7 and 
15 days (p<0.001). Similar effects were observed in cell proliferation, although signifi-
cant changes were obtained after prolonged exposures to high glucose (7 and 15 days; 
p<0.001). The proliferative response to the glucose-enriched environment was correlat-
ed to changes in the expression of PAC1, but not VPAC1 and VPAC2 receptors, as meas-
ured by quantitative real time PCR. These results were further confirmed by Western 
blot and immunofluorescence analyses. Interestingly, 100nM PACAP or VIP treatment 
significantly attenuated hyperglycemia-induced cell viability and proliferation after 7 
and 15 days. Taken together, our findings demonstrated that both PACAP and VIP pep-
tides exert an inhibitory activity on hyperglycemia-induced endothelial cell prolifera-
tion, and further suggest that the effect might be mediated by PAC1 receptors.
Key words
PACAP, VIP, endothelial cells, proliferation


Effects of PACAP and VIP on hyperglycemia-induced cell proliferation in murine microvascular endothelial cells

http://www.fupress.net/index.php/ijae/article/download/8785/8178

Effects of PACAP and VIP on hyperglycemia-induced cell proliferation in murine microvascular endothelial cells

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