Cataldi, Amelia

Cellini, Luigina

Di Giulio, Mara

D’Ercole, Simonetta

Rapino, Monica

Zara, Susi

Firenze University Press: E-Journals

IJAE Vol. 115, n. 1/2 (Supplement), 2010
180
Effects of HEMA on human gingival fibroblasts/clinical 
Streptococcus mitis strains co-cultured in vitro
Susi Zara1, Mara Di Giulio1, Simonetta D’Ercole2, Luigina Cellini1, Monica Rapino3, Amelia Cataldi4
1 Department of Drug Sciences, Unity of Chieti, Italy
2 Department of Oral Sciences, Unity of Chieti, Italy
3 Institute of Molecular Genetics CNR, Unity of Chieti, Italy
4 Department of Medicine and Ageing Sciences, “G. d’Annunzio” University, Chieti-Pescara, Italy
Recently dental implants have been modified in order to obtain an optimal integra-
tion between host tissues and implant material, besides the easy handle, sealing capabil-
ity and better aesthetic performance. In addition, one of the principal aims is the prepa-
ration of biomaterials not available for bacterial biofilm adhesion and colonization. Thus 
it is necessary to obtain standardized methods and protocols in order to better evaluate 
the efficiency of biomaterials used in dentistry to repair and replacement of previously 
damaged soft and hard tissue and/or organ by the study of biocompatibility.
Thus, the objective of this research has been the evaluation of the biological and 
molecular modifications occurring in human gingival fibroblasts/Streptococcus mitis co-
cultures “in vitro”, in the presence of HEMA (2-Hydroxyethil methacrylate), a monomer 
released by resinous dental materials, in order to reproduce the real situation existing in 
the oral cavity and to understand cellular and tissue reactions which can occur during 
interaction/integration between biomaterials/host tissue/microbial environment. 
Trypan blue exclusion test evidences a 50% of dead cells in HEMA treated cells when 
compared to untreated and cocultered ones.
In parallel the bacterial viability, evaluated by Live/Dead kit, is not modified in pres-
ence of HEMA and/or cells with respect to the controls.
The effects of HEMA and bacteria alone and in combination on the adhesion cells 
protein Pro-collagen I expression, involved in structural adhesion, carried out by means 
of immunofluorescence, evidences a physiological Pro-collagen I expression only in un-
treated samples, disappearing in the other experimental points. Surprisingly and unlike 
what has been revealed by trypan blue test, co-cultured samples treated with HEMA 
show a greater number of apoptotic nuclei when compared with HEMA treated ones. 
These data suggest that HEMA cytotoxic effect is more evident on eukaryotic cells than 
on prokaryotic ones and that it seems to be increased by bacteria presence. 
This work has been supported by PRIN 2007 Prof. Cataldi and CARICHIETI grants.


Effects of HEMA on human gingival fibroblasts/clinical Streptococcus mitis strains co-cultured in vitro

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Effects of HEMA on human gingival fibroblasts/clinical Streptococcus mitis strains co-cultured in vitro

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