CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
Peptides NAP and SAL attenuate human tau granular-shaped oligomers in vitro and in SH-SY5Y cells
Authors
S. Balalaie
B. Bolouri
+7 more
E. Fathi
A. Hemati
S. Karima
F.H. Katouli
R. Khodarahmi
F. Mokhtari
G. Riazi
Publication date
1 January 2016
Publisher
Abstract
Accumulation of human tau protein in the central nervous system is an outstanding feature of Alzheimer's Diseases and other tauopathies. Among the aggregate species, granular-shaped oligomers are recently reported as toxic species and associated with neuronal loss. Many interests have been focused on the reducing of these neurotoxic oligomers in tauopathies. It has been approved that peptides NAP (NAPVSIPQ) and SAL (SALLRSIPA), snipped from activity-dependent neurotrophic protein (ADNP) and activity-dependent neurotrophic factor (ADNF), protect brain against a wide range of toxins in neurodegenerative models. The neuroprotection mechanisms of NAP and SAL are under investigation. We report here that the aggregation pathway of human tau protein, in the presence of NAP or SAL, could be affected and moved toward the less granular-shaped species. Fluorophore binding assays, kinetic parameters and circular dichroism records, showed the presence of unusual aggregated species upon treatment with NAP or SAL, as well as hydrophobicity studies. Also, sizing and morphological investigation of aggregates, using dynamic scattered light and Atomic Force Microscopy, elucidated a significant reduction of granular-shaped oligomers under 50 nm. Furthermore, immunospecific detection of oligomers in SH-SY5Y cell line, using flow cytometric assessment supported our results. We conclude that one of the possible mechanisms of neuronal protection by NAP or SAL could be the attenuation of granular-shaped oligomeric tau under stress condition. © 201
Similar works
Full text
Available Versions
eprints Iran University of Medical Sciences
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:eprints.iums.ac.ir:3240
Last time updated on 10/10/2019